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首页> 外文期刊>Journal of applied toxicology >Automated swimming activity monitor for examining temporal patterns of toxicant effects on individual Daphnia magna
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Automated swimming activity monitor for examining temporal patterns of toxicant effects on individual Daphnia magna

机译：自动游泳活动监控器，用于检查对大型水蚤的毒性作用的时间模式

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摘要

Aquatic pollutants are often biologically active at low concentrations and impact on biota in combination with other abiotic stressors. Traditional toxicity tests may not detect these effects, and there is a need for sensitive high-throughput methods for detecting sublethal effects. We have evaluated an automated infra-red (IR) light-based monitor for recording the swimming activity of Daphnia magna to establish temporal patterns of toxicant effects on an individual level. Activity was recorded for 48 h and the sensitivity of the monitor was evaluated by exposing D. magna to the reference chemicals K2Cr2O7 at 15, 20 and 25 degrees C and 2,4-dichlorophenol at 20 degrees C. Significant effects (P < 0.001) of toxicant concentrations, exposure time and incubation temperatures were observed. At 15 degrees C, the swimming activity remained unchanged for 48 h at sublethal concentrations of K2Cr2O7 whereas activity at 20 and 25 degrees C was more biphasic with decreases in activity occurring after 12-18 h. A similar biphasic pattern was observed after 2,4-dichlorophenol exposure at 20 degrees C. EC50 values for 2,4-dichlorophenol and K2Cr2O7 determined fromautomated recording of swimming activity showed increasing toxicity with time corresponding to decreases in EC50 of 0.03-0.07 mg l(-1) h(-1). EC50 values determined after 48 h were comparable or lower than EC50 values based on visual inspection according to ISO 6341. The results demonstrated that the swimming activity monitor is capable of detecting sublethal behavioural effects that are toxicant and temperature dependent. The method allows EC values to be established at different time points and can serve as a high-throughput screening tool in toxicity testing. Copyright (C) 2015 John Wiley & Sons, Ltd.
机译：水生污染物通常在低浓度下具有生物活性，并与其他非生物胁迫源结合对生物群产生影响。传统的毒性测试可能无法检测到这些影响，因此需要灵敏的高通量方法来检测亚致死作用。我们已经评估了一种基于自动红外（IR）的监视器，用于记录大型蚤（Daphnia magna）的游泳活动，以建立单个水平上毒物作用的时间模式。记录48小时的活动并通过在15、20和25摄氏度下将D. magna暴露于参考化学物质K2Cr2O7和20摄氏度下的2,4-二氯苯酚中来评估监测器的灵敏度。显着影响（P <0.001）观察毒物浓度，暴露时间和温育温度。在15摄氏度时，在亚致死浓度的K2Cr2O7下，游泳活动保持48小时不变，而在20和25摄氏度时，游泳活动更呈两相，而在12-18小时后活动减少。在20°C下暴露于2,4-二氯苯酚后，观察到相似的两相模式。通过自动记录游泳活动而确定的2,4-二氯苯酚和K2Cr2O7的EC50值显示毒性随时间增加而增加，对应于EC3-0.03-0.07 mg / l的降低（-1）小时（-1）。 48小时后确定的EC50值与根据ISO 6341进行目视检查得出的EC50值相当或更低。结果表明，游泳活动监控器能够检测出有毒和温度依赖性的亚致死行为。该方法允许在不同时间点建立EC值，并且可以用作毒性测试中的高通量筛选工具。版权所有（C）2015 John Wiley＆Sons，Ltd.

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