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首页> 外文期刊>Journal of applied toxicology >Analysis of opiates, cocaine and metabolites in urine by high-performance liquid chromatography with diode array detection (HPLC-DAD).
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Analysis of opiates, cocaine and metabolites in urine by high-performance liquid chromatography with diode array detection (HPLC-DAD).

机译：高效液相色谱-二极管阵列检测（HPLC-DAD）分析尿液中的鸦片，可卡因和代谢物。

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摘要

An analytical method is proposed for the simultaneous determination of morphine, codeine, 6acetyl-morphine (MAM), cocaine, benzoylecgonine (BEG), cocaethylene, methadone and 2-ethylen-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in urine using high performance liquid chromatography coupled to a diode array detector (HPLC-DAD). The selection of working wavelengths is based on the highest chromatographic response for each component: 233 nm for cocaine, BEG and cocaethylene; 285 nm for morphine, codeine and MAM; and 292 nm for methadone and EDDP. The mobile phase, which is a mixture of acetonitrile and 0.02 M phosphate buffer at pH 6.53, was eluted in gradient mode through an XTerra((R)) RP-8 column (250 mm x 4.6 mm i.d., 5 microm particle size). After applying a solid-phase extraction procedure with Bond Elut Certify cartridges, the recoveries obtained were between 60% (EDDP) and 97% (cocaethylene). A good linearity of the method in the 0.1-10 microg mL(-1) range of urinary concentrations was obtained because the coefficient of correlation exceeded 0.99 for each drug. The precision and accuracy were quite good, with values of <7% and within the range +/- 6%, respectively. Finally, the proposed method was applied to 23 urine samples from fatal intoxications related to methadone, heroin and[sol ]or cocaine. Copyright (c) 2005 John Wiley & Sons, Ltd.
机译：提出了一种同时测定吗啡，可待因，6-乙酰基吗啡（MAM），可卡因，苯甲酰芽子碱（BEG），可卡乙烯，美沙酮和2-乙烯-1,5-二甲基-3,3-二苯基吡咯烷酮（EDDP）的分析方法使用高效液相色谱仪和二极管阵列检测器（HPLC-DAD）联用测定尿液中的尿素。工作波长的选择基于每种组分的最高色谱响应：可卡因，BEG和可卡乙烯233 nm;吗啡，可待因和MAM的285 nm;美沙酮和EDDP的波长为292 nm。流动相是乙腈和0.02 M磷酸盐缓冲液在pH 6.53中的混合物，通过X-8 RP-8色谱柱（250 mm x 4.6 mm i.d.，5微米粒径）以梯度洗脱。在使用Bond Elut Certify柱进行固相萃取后，回收率在60％（EDDP）和97％（可可乙烯）之间。该方法在尿液浓度为0.1-10 microg mL（-1）范围内获得了良好的线性，因为每种药物的相关系数均超过0.99。精度和准确性都非常好，其值分别小于7％和+/- 6％。最后，将所提出的方法应用于23例与美沙酮，海洛因和[或]可卡因有关的致命性中毒的尿液样品。版权所有（c）2005 John Wiley＆Sons，Ltd.

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