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首页> 外文期刊>Journal of applied toxicology >Benzo(a)pyrene inhibitsmigration and invasion of extravillous trophoblast HTR-8/SVneo cells via activation of the ERK and JNK pathway
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Benzo(a)pyrene inhibitsmigration and invasion of extravillous trophoblast HTR-8/SVneo cells via activation of the ERK and JNK pathway

机译：苯并（a）via通过激活ERK和JNK途径抑制绒毛滋养层HTR-8 / SVneo细胞的迁移和侵袭

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摘要

Benzo(a) pyrene (BaP) is a persistent organic pollutant (POP) that is a serious threat to human health. Numerous studies have shown that BaP causes adverse effects in pregnancy, but the mechanism remains unclear. The moderate invasion of trophoblast cells into the endometrium is an important factor during successful embryo implantation. The aim of this study was to investigate the effect and mechanism of BaP on the invasion and migration of trophoblast cells. HTR-8/SVneo cells were treated with different concentrations (1, 5, 10, 25, 50 and 100 mu M) of BaP. The invasion and migration of HTR-8/SVneo cells were observed after BaP treatment. The protein levels related to migration and invasion was detected by Western blot. The results confirmed that BaP inhibits the migration and invasion of extravillous trophoblast HTR-8/SVneo cells. Further investigations indicated that the protein levels of MMP-2, MMP-9 and E-cadherin in HTR-8/SVneo cells were changed by BaP treatment. Moreover, the data demonstrated that BaP activated the MAPK signaling pathway. Pretreatment with specific inhibitors of MAPK rescued BaP-induced change in the migration and invasion of HTR-8/SVneo cells. Taken together, our results indicated that BaP inhibits invasion and the migration of HTR-8/SVneo cells, which might cause a failure in early pregnancy. Copyright (C) 2015 John Wiley & Sons, Ltd.
机译：苯并（a）（（BaP）是一种持久性有机污染物（POP），对人类健康构成严重威胁。大量研究表明，BaP会在妊娠中引起不良反应，但机理尚不清楚。滋养层细胞适度侵入子宫内膜是成功胚胎植入过程中的重要因素。这项研究的目的是研究BaP对滋养细胞侵袭和迁移的作用和机制。用不同浓度（1、5、10、25、50和100μM）的BaP处理HTR-8 / SVneo细胞。 BaP处理后观察到HTR-8 / SVneo细胞的侵袭和迁移。通过蛋白质印迹检测与迁移和侵袭有关的蛋白质水平。结果证实BaP抑制绒毛外滋养细胞HTR-8 / SVneo细胞的迁移和侵袭。进一步的研究表明，通过BaP处理可以改变HTR-8 / SVneo细胞中MMP-2，MMP-9和E-cadherin的蛋白水平。而且，数据表明BaP激活了MAPK信号传导途径。用MAPK的特异性抑制剂预处理可以挽救BaP诱导的HTR-8 / SVneo细胞迁移和侵袭的变化。两者合计，我们的结果表明BaP抑制HTR-8 / SVneo细胞的侵袭和迁移，这可能会导致早期妊娠失败。版权所有（C）2015 John Wiley＆Sons，Ltd.

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