...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of applied toxicology >Use of proliferation tests to evaluate the effects of complexing agents on beryllium toxicity.
【24h】

Use of proliferation tests to evaluate the effects of complexing agents on beryllium toxicity.

机译：使用增殖试验评估络合剂对铍毒性的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Occupational exposure to beryllium may cause chronic beryllium disease (CBD), a granulomatous interstitial pneumonitis caused by a cell-mediated immune response with delayed hypersensitivity initiated by an electrostatic interaction with the MHC class II human leukocyte antigen (HLA). Increased research efforts focus on the development of a CBD treatment by chelation therapy. This work presents an in vitro evaluation of the beneficial effects of beryllium chelation with different organic substrates. We have used a standard beryllium lymphocyte proliferation test (BeLPT) adapted for mouse splenocytes. Three complexing agents, 4,5-dihydroxy-1,3-benzenedisulfonic acid (tiron), nitrilotripropionic acid (NTP) and nitrilotriacetic acid (NTA), were tested using different protocols of the splenocyte proliferation test (SPT). We studied their corrective effect (beryllium pre-exposed splenocytes), their protective effect (ligand pre-exposed splenocytes) and their combined effects at fixed Be:L ratio of 1:2, at fixed Be concentration and at fixed L concentration. We also studied the effect of tiron in preventing splenocyte sensitization to beryllium. All three complexing agents showed a corrective effect and proved efficient in the combined effects, except NTA in the fixed Be:L ratio. Only NTP and tiron showed a significant protection at lower beryllium concentrations, while NTA was not significant. Splenocytes pre-exposed to chelated beryllium did not show sensitization while splenocytes pre-exposed to beryllium were sensitized. We observed a strong correlation between the efficiency of the complexing agent and its affinity towards beryllium. Both tiron and NTP showed a similar affinity towards the beryllium ion that is 10(7) higher than that of NTA. Copyright (c) 2008 John Wiley & Sons, Ltd.
机译：职业接触铍可能会导致慢性铍病（CBD），这是一种肉芽肿性间质性肺炎，由细胞介导的免疫反应引起，并与MHC II类人类白细胞抗原（HLA）发生静电相互作用，导致迟发型超敏反应。越来越多的研究工作集中在通过螯合疗法开发CBD疗法上。这项工作提出了铍螯合与不同有机底物的有益作用的体外评估。我们已经使用了适用于小鼠脾细胞的标准铍淋巴细胞增殖测试（BeLPT）。使用脾细胞增殖测试（SPT）的不同方案测试了三种络合剂4,5,5-二羟基-1,3-苯二磺酸（tiron），次氮基三丙酸（NTP）和次氮基三乙酸（NTA）。我们研究了它们的校正作用（铍预先暴露的脾细胞），其保护作用（配体预先暴露的脾细胞）以及在固定的Be：L比为1：2，固定的Be浓度和固定的L浓度下的综合作用。我们还研究了铁蛋白在预防脾细胞对铍致敏方面的作用。除NTA固定的Be：L比例外，所有三种络合剂均显示出纠正作用并证明在合并作用中有效。在较低的铍浓度下，只有NTP和tiron表现出显着的保护作用，而NTA则不显着。预先暴露于螯合铍的脾细胞未显示致敏性，而预先暴露于铍的脾细胞被致敏。我们观察到络合剂的效率与其对铍的亲和力之间有很强的相关性。钛和NTP对铍离子的亲和力相似，比NTA高10（7）。版权所有（c）2008 John Wiley＆Sons，Ltd.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号