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首页> 外文期刊>Journal of applied toxicology >Chronic inhalation of biomass smoke is associated with DNA damage in airway cells: Involvement of particulate pollutants and benzene
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Chronic inhalation of biomass smoke is associated with DNA damage in airway cells: Involvement of particulate pollutants and benzene

机译：长期吸入生物质烟雾与气道细胞中的DNA损伤有关：颗粒污染物和苯的参与

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This study examined whether indoor air pollution from biomass fuel burning induces DNA damage in airway cells. For this, sputum cells were collected from 56 premenopausal rural women who cooked with biomass (wood, dung, crop residues) and 49 age-matched controls who cooked with cleaner liquefied petroleum gas. The levels of particulate matters with diameters of less than 10 and 2.5μm (PM10 and PM2.5) in indoor air were measured using a real-time aerosol monitor. Benzene exposure was monitored by measuring trans,trans-muconic acid (t,t-MA) in urine by HPLC-UV. DNA damage was examined by alkaline comet assay in sputum cells. Generation of reactive oxygen species (ROS) and level of superoxide dismutase (SOD) in sputum cells were measured by flow cytometry and spectrophotometry, respectively. Compared with controls, biomass users had 4 times higher tail percentage DNA, 37% more comet tail length and 5 times more Olive tail moment (p0.001) in inflammatory and epithelial cells in sputum, suggesting extensive DNA damage. In addition, women who cooked with biomass had 6 times higher levels of urinary t,t-MA and 2-fold higher levels of ROS generation concomitant with 28% depletion of SOD. Indoor air of biomass-using households had 2-4 times more PM10 and PM2.5 than that of controls. After controlling potential confounders, positive association was found between DNA damage parameters, particulate pollution, urinary t,t-MA and ROS. Thus, long-term exposure to biomass smoke induces DNA damage in airway cells and the effect was probably mediated, at least in part, by oxidative stress generated by inhaled particulate matter and benzene.
机译：这项研究检查了燃烧生物质燃料引起的室内空气污染是否会引起气道细胞DNA损伤。为此，从56名用生物质（木材，粪便，农作物残渣）烹饪的绝经前农村妇女和49名年龄更年长的对照组中收集痰细胞，这些对照组使用清洁的液化石油气烹饪。使用实时气溶胶监测仪测量室内空气中直径小于10和2.5μm（PM10和PM2.5）的颗粒物的水平。通过HPLC-UV测量尿液中的反式，反式粘康酸（t，t-MA）监测苯的暴露。通过碱性彗星试验检测痰细胞中的DNA损伤。通过流式细胞术和分光光度法分别测量痰细胞中活性氧（ROS）的产生和超氧化物歧化酶（SOD）的水平。与对照组相比，生物量使用者的痰液中炎症和上皮细胞的尾巴百分比DNA高4倍，彗星尾巴长度增加37％，橄榄尾巴矩增加5倍（p <0.001），表明DNA受到广泛破坏。此外，用生物质烹饪的妇女的尿中t，t-MA水平高6倍，ROS生成水平高2倍，同时SOD消耗28％。使用生物质的家庭的室内空气中PM10和PM2.5的含量是对照组的2-4倍。在控制了潜在的混杂因素之后，发现DNA损伤参数，微粒污染，尿液t，t-MA和ROS之间存在正相关。因此，长期暴露于生物质烟雾会引起气道细胞DNA损伤，这种影响可能至少部分是由吸入颗粒物和苯产生的氧化应激所介导的。

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