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首页> 外文期刊>Journal of applied toxicology >Palytoxin causes nonoxidative necrotic damage to PC12 cells in culture
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Palytoxin causes nonoxidative necrotic damage to PC12 cells in culture

机译：Palytoxin对培养物中的PC12细胞造成非氧化性坏死性损伤

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Palytoxin (PTX) is a potent marine toxin that causies serious damage to various tissues and organs. It has been reported to affect the transport of cations across the plasma membranes, which is commonly recognized as being the principal mechanism of its highly toxic action on mammals, including humans. However, although some marine toxins have been shown to cause toxic effects on the nervous system by interfering with the transmission of nerve impulses, the effect of PTX on neuronal cells has not yet been fully elucidated. Therefore, the toxic action of PTX on PC12 cells was examined as an in vitro model experiment to elucidate the neurotoxic properties of this toxin, and PTX was shown to reduce the viability of PC12 cells in a concentration-dependent manner. The cytotoxic action of PTX was not significantly altered by the presence of the antioxidant N-acetylcysteine and reduced-form glutathione in the cultures. Fluorescence staining of the cells and the electrophoretic analysis of genomic DNA showed that PTX failed to cause chromatin condensation and DNA fragmentation within the cells. On the other hand, the exposure to PTX caused positive staining of the cytoplasmic space of the cells with propidium iodide and the release of lactate dehydrogenase into the culture medium. Based on these observations, PTX is considered to cause cell death as a consequence of disrupting the plasma membranes, thus causing nonoxidative necrotic damage to PC12 cells. copy; 2012 John Wiley & Sons, Ltd.
机译：Palytoxin（PTX）是一种有效的海洋毒素，会对各种组织和器官造成严重损害。据报道影响阳离子跨质膜的运输，这通常被认为是其对包括人类在内的哺乳动物的高毒性作用的主要机理。然而，尽管已经显示出一些海洋毒素通过干扰神经冲动的传递而对神经系统产生毒性作用，但尚未完全阐明PTX对神经元细胞的作用。因此，为了阐明该毒素的神经毒性特性，通过体外模型实验检查了PTX对PC12细胞的毒性作用，并显示PTX以浓度依赖的方式降低PC12细胞的活力。在培养物中，抗氧化剂N-乙酰半胱氨酸和还原型谷胱甘肽的存在并未显着改变PTX的细胞毒性作用。细胞的荧光染色和基因组DNA的电泳分析表明，PTX未能引起细胞内的染色质浓缩和DNA片段化。另一方面，暴露于PTX导致碘化丙啶对细胞的细胞质空间进行正染色，并且乳酸脱氢酶释放到培养基中。基于这些观察，认为PTX由于破坏质膜而引起细胞死亡，从而对PC12细胞造成非氧化性坏死性损伤。复制; 2012年John Wiley＆Sons，Ltd.

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