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首页> 外文期刊>Journal of applied toxicology >Effects of cylindrospermopsin on the phagocytic cells of the common carp (Cyprinus carpio L.)
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Effects of cylindrospermopsin on the phagocytic cells of the common carp (Cyprinus carpio L.)

机译：cylindrospermopsin对鲤鱼吞噬细胞的影响

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摘要

Cylindrospermopsin is a cyanotoxin with cytotoxic activity. It is released into water during and after cyanobacterial water blooms and thus poses a threat to the health of fish. There is very little information available concerning the effects of the toxin on fish immune cells. In this study, we assessed the potential impact of cylindrospermopsin on the basic functions of phagocytic cells from common carp (Cyprinus carpio L.), including phagocytosis, reactive oxygen and nitrogen species production, and the structure of microfilaments and selected cytokine expression. Phagocytic cells, isolated from fish head kidneys, were exposed to the toxin at concentrations of 0.05, 0.1, 0.5 or 1 mu gml(-1), for up to 24h. Cytotoxicity, detected by lactate dehydrogenase release, was observed at the highest studied concentration. A decrease in phagocytic activity and changes in actin cytoskeletal structures were observed after the cell exposure to the toxin at 0.5 and 1 mu gml(-1). Moreover, at all tested concentrations, cylindrospermopsin increased the production of reactive oxygen and nitrogen species. It also evidently influenced the expression of genes of proinflammatory cytokines interleukin-1 and tumour necrosis factor- and, to a minor extent, anti-inflammatory transforming growth factor-, but had no effects on interleukin-10. The results indicated that the cyanotoxin cylindrospermopsin is able to modify basic features of carp phagocytic cells, which might result in adverse consequences for fish health. Copyright (c) 2015 John Wiley & Sons, Ltd.
机译：Cylindrospermopsin是一种具有细胞毒活性的氰毒素。它在蓝细菌水开花期间和之后释放到水中，从而对鱼类的健康构成威胁。关于毒素对鱼类免疫细胞影响的信息很少。在这项研究中，我们评估了cylindrospermopsin对普通鲤鱼（Cyprinus carpio L.）吞噬细胞基本功能的潜在影响，包括吞噬作用，活性氧和氮的产生，微丝的结构和细胞因子的表达。从鱼头肾脏中分离出来的吞噬细胞暴露于浓度为0.05、0.1、0.5或1μg·ml（-1）的毒素中长达24小时。在研究的最高浓度下观察到通过乳酸脱氢酶释放检测到的细胞毒性。在细胞暴露于0.5和1μggml（-1）的毒素后，吞噬活性降低，肌动蛋白细胞骨架结构发生变化。此外，在所有测试浓度下，圆柱精蛋白酶增加了活性氧和氮物质的产生。它也明显影响促炎细胞因子白介素-1和肿瘤坏死因子的基因表达，并在较小程度上影响抗炎转化生长因子的表达，但对白介素10没有影响。结果表明，氰毒素cylindrospermopsin能够改变鲤鱼吞噬细胞的基本特征，这可能对鱼类健康造成不利影响。版权所有（c）2015 John Wiley＆Sons，Ltd.

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