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首页> 外文期刊>Journal of applied toxicology >Functional expressions of adenosine triphosphate-binding cassette transporters during the development of zebrafish embryos and their effects on the detoxification of cadmium chloride and beta-naphthoflavone
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Functional expressions of adenosine triphosphate-binding cassette transporters during the development of zebrafish embryos and their effects on the detoxification of cadmium chloride and beta-naphthoflavone

机译：斑马鱼胚胎发育过程中三磷酸腺苷结合盒转运蛋白的功能表达及其对氯化镉和β-萘黄酮解毒的影响

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摘要

Adenosine triphosphate-binding cassette (ABC) transporters, including ABCB, ABCC and ABCG families represent general biological defenses against environmental toxicants in varieties of marine and freshwater organisms, but their physiological functions at differential developmental stages of zebrafish embryos remain undefined. In this work, functional expressions of typical ABC transporters including P-glycoprotein (Pgp), multiresistance associated protein 1 (Mrp1) and Mrp2 were studied in zebrafish embryos at 4, 24, 48 and 72h post-fertilization (hpf). As a result, both the gene expressions and activities of Pgp and Mrps increased with the development of embryos. Correspondingly, 4-72 hpf embryos exhibited an increased tolerance to the toxicity caused by cadmium chloride (CdCl2) and beta-naphthoflavone (BNF) with time. Such a correlation was assumed caused by the involvement of ABC transporters in the detoxification of chemicals. In addition, the assumption was supported by the fact that model efflux inhibitors of Pgp and Mrps such as reversine 205 and MK571 significantly inhibited the efflux of toxicants and increased the toxicity of Cd and BNF in zebrafish embryos. Moreover, exposure to CdCl2 and BNF induced the gene expressions of Pgp and Mrp1 in 72hpf embryos. Thus, functional expressions of Pgp and Mrps increased with the development of zebrafish embryos, which could cause an increasing tolerance of zebrafish embryos to CdCl2 and BNF. Copyright (C) 2015 John Wiley & Sons, Ltd.
机译：包括ABCB，ABCC和ABCG家族在内的三磷酸腺苷结合盒（ABC）转运蛋白代表了各种海洋和淡水生物对环境毒物的一般生物防御作用，但是它们在斑马鱼胚胎发育的不同阶段的生理功能仍然不确定。在这项工作中，研究了受精后4、24、48和72h在斑马鱼胚胎中典型的ABC转运蛋白的功能表达，包括P糖蛋白（Pgp），多抗相关蛋白1（Mrp1）和Mrp2。结果，Pgp和Mrps的基因表达和活性都随着胚胎的发育而增加。相应地，随着时间的推移，4-72 hpf胚胎对由氯化镉（CdCl2）和β-萘黄酮（BNF）引起的毒性的耐受性增强。假定这种相关性是由于ABC转运蛋白参与了化学物质的排毒。此外，这一假设得到了以下事实的支持：Pgp和Mrps的模型外排抑制剂，例如反转酶205和MK571，显着抑制了有毒物质的外排，并增加了Cd和BNF在斑马鱼胚胎中的毒性。此外，暴露于CdCl2和BNF可以诱导72hpf胚胎中Pgp和Mrp1的基因表达。因此，Pgp和Mrps的功能性表达随斑马鱼胚胎的发育而增加，这可能导致斑马鱼胚胎对CdCl2和BNF的耐受性增加。版权所有（C）2015 John Wiley＆Sons，Ltd.

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