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首页> 外文期刊>Journal of applied toxicology >Kinetic analysis of hexose transport to determine the mechanism of amygdalin and prunasin absorption in the intestine.
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Kinetic analysis of hexose transport to determine the mechanism of amygdalin and prunasin absorption in the intestine.

机译：己糖转运的动力学分析，以确定苦杏仁苷和李宁在肠道中的吸收机理。

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摘要

Evidence is accumulating that glucose-conjugated compounds may be carried across the gut mucosa via the epithelial sodium-dependent monosaccharide transporter SGLT1. A modification of the everted intestinal sac technique was utilized to study the transport of the cyanogenic glycoside amygdalin (D-mandelonitrile beta-D-gentiobioside) and its metabolite D-mandelontrile beta-D-glucoside (prunasin). Everted sacs of rat jejunum and ileum were bathed in isotonic oxygenated sodium chloride-potassium phosphate buffer containing 2.8 microCi D-[(3)H]-mannose and 0.187 microCi D-[(14)C]-glucose. For treatment groups, buffers contained phloridzin, galactose, amygdalin or prunasin. The rate constant (k) for the transport process was calculated. Compared with the control (n = 33), phloridzin (n = 25) significantly reduced the rate constants of both D-[(14)C]-glucose and D-[(3)H]-mannose. Substitution of sodium with choline and incremental galactose treatments similarly reduced D-[(14)C]-glucose influx, indicating that a fraction of the transport is carrier-mediated. Treatment with amygdalin did not significantly affect the rate constants of D-[(14)C]-glucose or D-[(3)H]-mannose transport. However, treatment with 1 mM prunasin (n = 16) did reduce the influx of D-[(14)C]-glucose without affecting D-[(3)H]-mannose values. This is consistent with the reports finding that glycoside absorption may be mediated by SGLT1.
机译：越来越多的证据表明，结合葡萄糖的化合物可能通过上皮钠依赖性单糖转运蛋白SGLT1穿过肠道粘膜。利用外翻小肠囊囊技术的改进方法来研究生氰糖苷苦杏仁苷（D-扁桃腈β-D-龙胆总糖苷）及其代谢产物D-扁桃腈β-D-葡糖苷（普鲁西星）的转运。将大鼠空肠和回肠的外翻囊浸入含有2.8 microCi D-[（3）H]-甘露糖和0.187 microCi D-[（14）C]-葡萄糖的等渗氧化氯化钠-磷酸钾缓冲液中。对于治疗组，缓冲液中含有Phloridzin，半乳糖，苦杏仁苷或Prunasin。计算出运输过程的速率常数（k）。与对照组（n = 33）相比，phloridzin（n = 25）显着降低了D-[（14）C]-葡萄糖和D-[（3）H]-甘露糖的速率常数。用胆碱和增量半乳糖处理取代钠同样减少了D-[（14）C]-葡萄糖的流入，表明一部分转运是由载体介导的。苦杏仁苷治疗不会显着影响D-[（（14）C]-葡萄糖或D-[（3）H]-甘露糖转运的速率常数。但是，用1 mM的胰蛋白酶（n = 16）的治疗确实减少了D-[（14）C]-葡萄糖的流入，而不影响D-[（3）H]-甘露糖的值。这与发现糖苷吸收可能由SGLT1介导的报道一致。

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