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首页> 外文期刊>Journal of Basic Microbiology: An International Journal on Morphology, Physiology, Genetics, and Ecology of Microorganisms >Identification and biocontrol efficacy of Streptomyces miharaensis producing filipin III against Fusarium wilt
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Identification and biocontrol efficacy of Streptomyces miharaensis producing filipin III against Fusarium wilt

机译：产硫链球菌III原链霉菌对枯萎病的鉴定和生防功效

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摘要

A number of bacterial strains were isolated from the internal tissue of Trapa japonica. Of these, strain KPE62302H, which had a 16S rDNA sequence identical to that of Streptomyces miharaensis showed antifungal activity against several plant pathogens. Treatment of seeds with strain KPE62302H induced a significant reduction in the incidence of Fusarium wilt in tomato plants compared with untreated controls. An antifungal substance (FP-1) was purified from the culture extract of strain KPE62302H using C18 flash and Sephadex LH-20 column chromatography and reverse phase HPLC. Extensive spectrometric analysis using MS and NMR identified this as filipin III. FP-1 inhibited the mycelial growth of plant pathogenic fungi such as Alternaria mali, Aspergillus niger, Colletotrichum gloeosporioides, C. orbiculare, Cylindrocarpon destructans, Diaporthe citiri, Fusarium oxysporum at 1-10 μg ml -1 and also markedly inhibited the development of Fusarium wilt caused by F. oxysporum f.sp. lycopersici in tomato plants by treatment with 10 μg ml -1 under greenhouse conditions. The efficacy of FP-1 against Fusarium wilt was comparable to that of the synthetic fungicide benomyl. An egfp -tagged strain of KPE62302H confirmed its ability to colonize tomato plants.
机译：从日本pa的内部组织中分离出许多细菌菌株。其中，菌株KPE62302H具有与mihara链霉菌相同的16S rDNA序列，显示出对几种植物病原体的抗真菌活性。与未处理的对照相比，用KPE62302H菌株处理种子诱导番茄植株枯萎病的发生率显着降低。使用C18闪速和Sephadex LH-20柱色谱法和反相HPLC从菌株KPE62302H的培养物提取物中纯化抗真菌物质（FP-1）。使用MS和NMR进行的广泛光谱分析确定为菲律宾血脂III。 FP-1以1-10μg/ ml -1抑制植物病原性真菌的菌丝生长，例如马氏链球菌，黑曲霉，球孢炭疽菌，球形念珠菌，Cylindrocarpon destructans，Diaporthe citiri，尖孢镰刀菌（Fusarium oxysporum）的菌丝生长，并显着抑制镰刀菌的发育枯萎病菌引起的枯萎在温室条件下用10μgml -1处理番茄植物中的lycopersici。 FP-1对抗枯萎病的功效与合成杀真菌剂苯菌灵的功效相当。带有egfp标签的KPE62302H菌株证实了其在番茄植物上定殖的能力。

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