Artificial metalloenzymes are expected to combine enzymatic selectivity with the broad range of catalytic motifs provided by homogeneous catalysts. Using specifically designed metal-conjugated affinity labels to introduce metal centres into the binding pocket of cysteine proteases, we were able to overcome the lack of structural definition, which tends to hamper catalytic selectivity. Experimental results proof, that the protein ligand induces enantioselectivities. The novel modular platform and the in situ protocol allow fast generation of diverse libraries of organometallic enzyme hybrid catalysts (see figure) [1].
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