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首页> 外文期刊>Journal of biological inorganic chemistry: JBIC: a publication of the Society of Biological Inorganic Chemistry >His-containing plant metallothioneins: comparative study of divalent metal-ion binding by plant MT3 and MT4 isoforms
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His-containing plant metallothioneins: comparative study of divalent metal-ion binding by plant MT3 and MT4 isoforms

机译：含His的植物金属硫蛋白：植物MT3和MT4同工型对二价金属离子结合的比较研究

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摘要

Metallothioneins (MTs) are a superfamily of Cys-rich, low-molecular weight metalloproteins that bind heavy metal ions. These cytosolic metallopeptides, which exist in most living organisms, are thought to be involved in metal homeostasis, metal detoxification, and oxidative stress protection. In this work, we characterise the Zn(II)-and Cd(II)-binding abilities of plant type 3 and type 4 MTs identified in soybean and sunflower, both of them being His-containing peptides. The recombinant metal-MT complexes synthesised in Zn(II) or Cd(II)-enriched Escherichia coli cultures have been analysed by ESI-MS, and CD, ICP-AES, and UV spectroscopies. His-to-Ala type 3 MT mutants have also been constructed and synthesised for the study of the role of His in divalent metal ion coordination. The results show comparable divalent metal-binding capacities for the MTs of type 3, and suggest, for the first time, the participation of their conserved C-term His residues in metal binding. Interesting features for the Zn(II)-binding abilities of type 4 MTs are also reported, as their variable His content may be considered crucial for their biological performance.
机译：金属硫蛋白（MTs）是结合重金属离子的富含Cys的低分子量金属蛋白的超家族。这些胞质金属肽存在于大多数活生物体中，被认为与金属稳态，金属解毒和氧化应激保护有关。在这项工作中，我们表征了在大豆和向日葵中鉴定出的植物3型和4型MT的Zn（II）和Cd（II）结合能力，它们都是含His的肽。通过ESI-MS，CD，ICP-AES和UV光谱分析了在富含Zn（II）或Cd（II）的大肠杆菌培养物中合成的重组金属MT复合物。还构建并合成了His-to-Ala 3型MT突变体，用于研究His在二价金属离子配位中的作用。结果表明，3型MT具有相当的二价金属结合能力，并首次表明它们的保守C末端His残基参与金属结合。还报道了4型MT的Zn（II）结合能力的有趣特征，因为它们的变量His含量可能被认为对其生物学性能至关重要。

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