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首页> 外文期刊>Journal of Applied Ichthyology >Genome size variation estimated by flow cytometry in Acipenser mikadoi, Huso dauricus in relation to other species of Acipenseriformes
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Genome size variation estimated by flow cytometry in Acipenser mikadoi, Huso dauricus in relation to other species of Acipenseriformes

机译：通过流式细胞术估计的米氏中华cip，胡氏da与其他种类的中华cip的基因组大小变异

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摘要

In Acipenseriformes, extant species can be categorized into three ploidy groups based on their genome size inferred from chromosome numbers and / or nuclear DNA content. However, genome size is still inconclusive in some sturgeon species. Here, we reported genome size of Acipenser mikadoi, Huso dauricus, other seven sturgeon and one paddlefish species quantified by nuclear DNA content flow cytometry. We also measured DNA content of artificially propagated progenies of A. mikadoi and H. dauricus, and progenies from several hybrids using eggs of the two sturgeon species and the hybrid bester. Nuclear DNA content of A. mikadoi was 8.2 pg and thus this species was categorized into functional tetraploid or evolutionary octaploid species like closely related A. medirostris. Although H. dauricus has been considered to be functional diploid or evolutionary tetraploid species based on previous results, this species had 8.3 pg DNA content and was classified into the functional tetraploid or evolutionary octaploid group which includes A. mikadoi. Among artificially propagated A. mikadoi, high frequencies of triploid individuals (45%) and a little portion of tetraploid individuals (1.5%) were detected. The occurrence of polyploid larvae was also detected in hybrid progenies using eggs of A. mikadoi. All 1-year-old survivors (n = 3) of artificially propagated H. dauricus were triploid. The spontaneous occurrence of such genetic triploid and tetraploid progenies suggests that polyploidization event presumably occurs even at present and may cause intraspecific variation of genome size in Acipenseridae.
机译：在Acipenseriformes中，根据从染色体数目和/或核DNA含量推断出的基因组大小，现存物种可分为三个倍性组。但是，在某些st鱼物种中，基因组大小仍然不确定。在这里，我们报道了通过核DNA含量流式细胞仪量化的Acipenser mikadoi，Huso dauricus，其他7个urge鱼和1个pad鱼的基因组大小。我们还测量了人工繁殖的A. mikadoi和H. dauricus子代以及使用两种using鱼卵和杂种bester的几种杂种后代的DNA含量。 mikadoi的核DNA含量为8.2 pg，因此该物种被分类为功能性四倍体或进化八倍体物种，如紧密相关的A. medirostris。尽管基于先前的结果已将H. dauricus视为功能性二倍体或进化四倍体物种，但该物种具有8.3 pg DNA含量，并被分类为功能性四倍体或进化八倍体组，其中包括mikadoi。在人工繁殖的A. mikadoi中，检测到高频率的三倍体个体（45％）和一小部分四倍体个体（1.5％）。使用mikadoi的卵在杂交后代中也检测到多倍体幼虫的发生。人工繁殖的H. dauricus的所有1岁存活者（n = 3）都是三倍体。这种遗传三倍体和四倍体后代的自发出现表明多倍体化事件大概甚至在目前就已经发生，并且可能引起A科的基因组大小的种内变化。

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