首页> 外文期刊>Journal of Applied Ichthyology >Ultrastructure of grey mullet (Mugil cephalus, Linnaeus, 1758) spermatozoa as revealed from light, scanning and transmission electron microscopy
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Ultrastructure of grey mullet (Mugil cephalus, Linnaeus, 1758) spermatozoa as revealed from light, scanning and transmission electron microscopy

机译:从光,扫描和透射电镜观察到的鱼(Mugil cephalus,Linnaeus,1758)精子的超微结构

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摘要

Spermatozoa morphology and fine structure were studied in the grey mullet, Mugil cephalus using light, scanning and transmission electron microscopy. Light microscopy observations indicate a semi-cystic type of spermatogenesis in the testis. The electron microscopy micrograph showed that the spermatozoon of M. cephalus is uniflagellated (total length 5.78 +/- 1.26 mu m), differentiated into an ovoid-shaped head without acrosome (1.80 +/- 0.35 mu m in length and 1.91 +/- 0.30 mu m in diameter), with a short midpiece and a long cylindrical flagellar tail (length 3.60 +/- 0.50 mu m). The midpiece is characterized by the presence of four to five vacuoles, a cytoplasmic canal, two centriole and two spherical mitochondria having a flat type of cristae. Chromatin granules of the nucleus form an electron-dense homogeneous mass. The flagellum consists of nine peripheral microtubules and a central pair (9 + 2) surrounded by the plasma membrane with side fins. The results confirm that spermatozoa of M. cephalus are perciform or teleostean type II. Information generated from the present study will be useful in taxonomic classification, cryopreservation and breeding work.
机译:利用光镜,扫描电镜和透射电镜观察了鱼(Mugil cephalus)的精子形态和精细结构。光学显微镜观察表明睾丸是半囊性的生精类型​​。电镜观察显示,头孢分支杆菌的精子呈单鞭毛状(全长5.78 +/- 1.26μm),分化为无顶体的卵形头(长1.80 +/- 0.35μm,1.91 +/-)直径0.30微米),带有短的中段和长的圆柱形鞭毛尾(长度3.60 +/- 0.50微米)。该中段的特征在于存在四到五个液泡,胞质管,两个中心细胞和两个具有扁平type的球形线粒体。核的染色质颗粒形成电子密集的均质物质。鞭毛由九个外围微管和一个中央对(9 + 2)组成,中央对(9 + 2)被质膜与侧鳍包围。结果证实,头颅分枝杆菌的精子为periform或teleostean II型。从本研究中获得的信息将有助于分类学分类,冷冻保存和育种工作。

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