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首页> 外文期刊>The journal of asthma >Elevated interferon regulatory factor 4 levels in patients with allergic asthma
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Elevated interferon regulatory factor 4 levels in patients with allergic asthma

机译：过敏性哮喘患者干扰素调节因子4水平升高

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Objective. Allergic asthma is a common inflammatory disease regulated by the T helper (Th) cells. Interferon regulatory factor 4 (IRF4) plays an important role in the differentiation of Th cells. This study investigated whether IRF4 is involved in the systemic immune responses in allergic asthma patients. Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from allergic asthmatics (n = 29) and healthy controls (n = 12). The mRNA and protein levels of IRF4 in PBMCs were measured by quantitative RT-PCR and western blotting. The frequencies of Th1, Th2, and Th17 cells were analyzed by flow cytometry. The related cytokine (interferon-γ, interleukin-4 (IL-4), and IL-17) concentrations in plasma and culture supernatants were measured by ELISA. The levels of GATA binding protein 3 (GATA3), retinoic acid-related orphan receptor γt (RORγt), and forkhead box P3 (FOXP3), the master transcription factors controlling Th2, Th17, and T regulatory cells differentiation, respectively, were examined by quantitative RT-PCR. Results. The levels of IRF4 were elevated in allergic asthmatics compared with those in healthy controls. The frequencies of Th2 and Th17 cells as well as the concentrations of Th2- and Th17-related cytokines were higher in plasma from asthma patients than those from healthy controls; similar results were observed in culture supernatants. IRF4 mRNA levels were positively correlated with GATA3, RORγt, and FOXP3 mRNA levels in allergic asthmatics but not in healthy controls. Conclusions. Our results suggested that IRF4 may play a role in the systemic inflammation of allergic asthma patients by facilitating the differentiation of Th2 and Th17 cells at least at the transcriptional level.
机译：目的。过敏性哮喘是由T辅助（Th）细胞调节的常见炎性疾病。干扰素调节因子4（IRF4）在Th细胞分化中起重要作用。这项研究调查了IRF4是否参与过敏性哮喘患者的全身免疫反应。方法。从过敏性哮喘（n = 29）和健康对照（n = 12）中分离出外周血单个核细胞（PBMC）。通过定量RT-PCR和蛋白质印迹法检测PBMC中IRF4的mRNA和蛋白水平。通过流式细胞仪分析Th1，Th2和Th17细胞的频率。通过ELISA测量血浆和培养上清液中的相关细胞因子（干扰素-γ，白介素-4（IL-4）和IL-17）的浓度。 GATA结合蛋白3（GATA3），视黄酸相关孤儿受体γt（RORγt）和叉头盒P3（FOXP3）的水平分别通过控制Th2，Th17和T调节性细胞分化的主要转录因子进行检测定量RT-PCR。结果。与健康对照组相比，过敏性哮喘患者中IRF4的水平升高。哮喘患者血浆中Th2和Th17细胞的频率以及Th2和Th17相关细胞因子的浓度高于健康人。在培养上清液中观察到相似的结果。在过敏性哮喘患者中，IRF4 mRNA水平与GATA3，RORγt和FOXP3 mRNA水平呈正相关，而在健康对照组中则不相关。结论。我们的结果表明，IRF4至少通过在转录水平上促进Th2和Th17细胞的分化，可能在过敏性哮喘患者的全身炎症中起作用。

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