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首页> 外文期刊>Journal of Agriculture of the University of Puerto Rico >Morphological, pathogenic and molecular characterization of Phoma spp. isolated from onion field soils in Puerto Rico
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Morphological, pathogenic and molecular characterization of Phoma spp. isolated from onion field soils in Puerto Rico

机译：Phoma spp的形态，致病性和分子特征。从波多黎各洋葱田土壤中分离

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摘要

Thirteen Phoma spp. isolates collected during a survey conducted in onion field soils in Santa Isabel, Puerto Rico, were examined on the basis of morphology, pathogenicity and molecular characteristics. Twelve isolates were identified as Phoma putaminum Speg. and one isolate as an atypical Phoma macrostoma var. incolorata (section Phyllostictoides).Trts is the first report of P. putaminum and P. macrostoma var. incolorata for Puerto Rico and the Caribbean. In vitro, Phoma putaminum isolates were pathogenic to onion cvs. Mercedes and Excalibur, resulting in necrosis of young bulbs and roots seven days after inoculation. Disease incidence caused by P. putaminum was higher in cultivar Mercedes, ranging from 75 to 100%, than in P. macrostoma var. incolorata (0 to 25%). No symptoms were observed on cultivar Excalibur inoculated with P. macrostoma var. incolorata or on control plants. In vitro inoculations of commercial onion bulbs and field inoculations of roots failed to reproduce symptoms, thus showing that P. putaminum and P. macrostoma var. incolorata are weak pathogens. Sequence size of the nuclear internal transcribed spacer (ITS) of ribosomal DNA (rDNA) gene ranged from 458 to 610 base pairs (bp) for P. putaminum and was 456 bp for P. macrostoma var. incolorata isolate. Analysis of rDNA ITS region by PCR-RFLP showed that restriction enzyme, Hindlll, among other restriction enzymes evaluated (Alul, EcoRI, Clal and Seal), differentiate between P. putaminum and P. macrostoma var. incolorata isolates.
机译：十三个Phoma spp。在形态，致病性和分子特征的基础上，对在波多黎各圣伊莎贝尔洋葱田土壤中进行的调查中收集到的分离株进行了检查。十二个分离物被鉴定为腐烂腐烂霉。另一种是非典型的Phoma macrostoma var。 incolorata（Phyllostictoides部分）.Trt是腐烂假单胞菌和大型假单胞菌变种的首次报道。 incolorata为波多黎各和加勒比海地区。在体外，腐烂腐殖质分离株对洋葱cvs具有致病性。 Mercedes和Excalibur，接种后7天导致小鳞茎和根坏死。腐病假单胞菌引起的疾病发生率在梅赛德斯品种中较高，从75％到100％不等。 incolorata（0至25％）。在接种了P. macrostoma var的Excalibur品种上未观察到症状。 incolorata或对照植物。商业洋葱鳞茎的体外接种和根部的野外接种均未能重现症状，因此表明恶臭假单胞菌和大假单胞菌变种。 incolorata是弱病原体。核糖体DNA（rDNA）基因的核内转录间隔区（ITS）的序列大小在P. putaminum的范围为458至610个碱基对（bp），在P. macrostoma var的范围为456 bp。 incolorata分离物。通过PCR-RFLP分析rDNA ITS区域显示，限制酶HindIII与其他评估的限制酶（Alul，EcoRI，Clal和Seal）相比，区分了腐烂假单胞菌和巨大假单胞菌。 incolorata分离物。

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