首页> 外文期刊>Journal of Agriculture of the University of Puerto Rico >Expression of pregnancy-specific beta-1-glycoprotein, aromatase and nitric oxide synthase in bovine sperm1
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Expression of pregnancy-specific beta-1-glycoprotein, aromatase and nitric oxide synthase in bovine sperm1

机译:妊娠特异性β-1-糖蛋白,芳香化酶和一氧化氮合酶在牛sperm1中的表达

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摘要

In search of alternative indicators of fertility in bulls, this research evaluated the effects of breed, type of cattle and age on the relative expression of the genes aromatase, pregnancy-specific beta-1-glycoprotein (PSG1), and nitric oxide synthase(NOS) in bovine semen. Sperm from Jersey, Holstein, Brahman, Brangus, Charoláis, Charbray, Senepol, and Simmental bulls was collected and the extracted mRNA was analyzed for expression of the genes using real time polimerase chain reaction (RT-PCR). The relative expression of aromatase was lower (P < 0.07) in sperm from Jersey bulls (0.89) than in bulls of all other breeds (average 1.01). The relative expression of PGS1 was greater (P > 0.02) in sperm from dairy (1.00) than from beef bulls (0.96). Agedid not affect relative expression of the genes under study. Additionally, in data from 31 bulls, expression of NOS was positively correlated with scrotal circumference (r = 0.31; P < 0.08). As far as we know, these are the first results showing that the genes aromatase, PSG1 and NOS can be detected in bull sperm and may have implications as molecular markers for bull fertility.
机译:为了寻找公牛育性的替代指标,本研究评估了品种,牛的类型和年龄对芳香酶,妊娠特异性β-1-糖蛋白(PSG1)和一氧化氮合酶(NOS)基因相对表达的影响。 )在牛精液中。收集来自泽西岛,霍尔斯坦,婆罗门,布朗格斯,夏洛来牛,夏布雷,塞内波尔和西门塔尔牛的精子,并使用实时聚合酶链反应(RT-PCR)分析提取的mRNA的基因表达。泽西公牛的精子中芳香化酶的相对表达较低(P <0.07)(0.89),低于所有其他品种的公牛(平均1.01)。乳制品(1.00)的精子中PGS1的相对表达大于牛(0.96)的PGS1(P> 0.02)。 Agedid不会影响所研究基因的相对表达。此外,在来自31头公牛的数据中,NOS的表达与阴囊周长呈正相关(r = 0.31; P <0.08)。据我们所知,这些是第一个结果,表明在牛精子中可以检测到芳香酶,PSG1和NOS基因,并且可能作为牛繁殖力的分子标记。

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