...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Bone and Joint Surgery. American Volume >The effects of exogenous basic fibroblast growth factor on intrasynovial flexor tendon healing in a canine model.
【24h】

The effects of exogenous basic fibroblast growth factor on intrasynovial flexor tendon healing in a canine model.

机译：外源性碱性成纤维细胞生长因子对犬模型滑膜内屈肌腱愈合的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Studies have demonstrated that flexor tendon repair strength fails to increase in the first three weeks following suturing of the tendon, a finding that correlates closely with the timing of many clinical failures. The application of growth factors holds promise for improving the tendon-repair response and obviating failure in the initial three weeks. METHODS: The effects of basic fibroblast growth factor on flexor tendon healing were evaluated with use of a canine model. Operative repair followed by the sustained delivery of basic fibroblast growth factor, at two different doses, was compared with operative repair alone. Histological, biochemical, and biomechanical methods were used to evaluate the tendons twenty-one days after repair. RESULTS: Vascularity, cellularity, and adhesion formation were increased in the tendons that received basic fibroblast growth factor as compared with the tendons that received operative repair alone. DNA concentration was increased in the tendons that received 1000 ng of basic fibroblast growth factor (mean and standard deviation, 5.7 +/- 0.7 mug/mg) as compared with the tendons that received 500 ng of basic fibroblast growth factor (3.8 +/- 0.7 mug/mg) and the matched control tendons that received operative repair alone (4.5 +/- 0.9 mug/mg). Tendons that were treated with basic fibroblast growth factor had a lower ratio of type-I collagen to type-III collagen, indicating increased scar formation compared with that seen in tendons that received operative repair alone (3.0 +/- 1.6 in the group that received 500-ng basic fibroblast growth factor compared with 4.3 +/- 1.0 in the paired control group that received operative repair alone, and 3.4 +/- 0.6 in the group that received 1000-ng basic fibroblast growth factor compared with 4.5 +/- 1.9 in the paired control group that received operative repair alone). Consistent with the increases in adhesion formation that were seen in tendons treated with basic fibroblast growth factor, the range of motion was reduced in the group that received the higher dose of basic fibroblast growth factor than it was in the paired control group that received operative repair alone (16.6 degrees +/- 9.4 degrees in the group that received 500 ng basic fibroblast growth factor, 13.4 degrees +/- 6.1 degrees in the paired control group that received operative repair alone, and 29.2 degrees +/- 5.8 degrees in the normal group [i.e., the group of corresponding, uninjured tendons from the contralateral forelimb]; and 15.0 degrees +/- 3.8 degrees in the group that received 1000 ng basic fibroblast growth factor, 19.3 degrees +/- 5.5 degrees in the paired control group that received operative repair alone, and 29.0 degrees +/- 8.8 degrees in the normal group). There were no significant differences in tendon excursion or tensile mechanical properties between the groups that were treated with basic fibroblast growth factor and the groups that received operative repair alone. CONCLUSIONS: Although basic fibroblast growth factor accelerated the cell-proliferation phase of tendon healing, it also promoted neovascularization and inflammation in the earliest stages following the suturing of the tendon. Despite a substantial biologic response, the administration of basic fibroblast growth factor failed to produce improvements in either the mechanical or functional properties of the repair. Rather, increased cellular activity resulted in peritendinous scar formation and diminished range of motion.
机译：背景：研究表明，在缝合肌腱后的前三周，屈肌腱修复强度未能提高，这一发现与许多临床衰竭的发生时间密切相关。生长因子的应用有望改善肌腱修复反应并在开始的三周内消除失败。方法：使用犬模型评估碱性成纤维细胞生长因子对屈肌腱愈合的影响。将手术修复后持续递送两种不同剂量的碱性成纤维细胞生长因子与单纯手术修复进行了比较。使用组织学，生化和生物力学方法评估修复后21天的肌腱。结果：与仅接受手术修复的肌腱相比，接受碱性成纤维细胞生长因子的肌腱的血管，细胞性和粘附形成增加。与接受500 ng碱性成纤维细胞生长因子（3.8 +/-）的肌腱相比，接受1000 ng碱性成纤维细胞生长因子的肌腱的DNA浓度增加（平均值和标准偏差，5.7 +/- 0.7 mug / mg）。 0.7杯/毫克）和匹配的对照肌腱，仅接受手术修复（4.5 +/- 0.9杯/毫克）。用碱性成纤维细胞生长因子治疗的肌腱的I型胶原蛋白与III型胶原蛋白的比率较低，这表明与仅接受手术修复的肌腱相比，瘢痕形成增加了（接受手术的组为3.0 +/- 1.6） 500 ng碱性成纤维细胞生长因子，而配对接受单独手术修复的对照组为4.3 +/- 1.0，而接受1000 ng碱性成纤维细胞生长因子的对照组为3.4 +/- 0.6，相比之下则为4.5 +/- 1.9在仅接受手术修复的配对对照组中）。与用碱性成纤维细胞生长因子治疗的肌腱中粘附形成增加一致，与接受手术修复的配对对照组相比，接受碱性成纤维细胞生长因子剂量较高的组的运动范围减小了单独使用（接受500 ng基本成纤维细胞生长因子的组为16.6度+/- 9.4度，单独接受手术修复的配对对照组为13.4度+/- 6.1度，正常情况下为29.2度+/- 5.8度组[即，对侧前肢相应的，未受伤的肌腱组];接受1000 ng碱性成纤维细胞生长因子的组为15.0度+/- 3.8度，配对对照组为19.3度+/- 5.5度，仅接受手术修复，正常组为29.0度+/- 8.8度）。在用碱性成纤维细胞生长因子治疗的组和仅接受手术修复的组之间，肌腱偏移或拉伸机械性能没有显着差异。结论：尽管碱性成纤维细胞生长因子加速了肌腱愈合的细胞增殖阶段，但在肌腱缝合后的最早阶段也促进了新血管形成和炎症。尽管有重要的生物学反应，但碱性成纤维细胞生长因子的给药未能在修复的机械或功能性质上产生改善。而是，增加的细胞活性导致周围的疤痕形成和运动范围减小。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号