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首页> 外文期刊>Journal of Bioactive and Compatible Polymers >Plasma Protein Adsorption and Platelet Adhesion on Heparin-immobilized Polyurethane Films
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Plasma Protein Adsorption and Platelet Adhesion on Heparin-immobilized Polyurethane Films

机译：肝素固定的聚氨酯膜上血浆蛋白的吸附和血小板的粘附

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摘要

Polyurethane surfaces were modified by covalent immobilization of different molecular weight heparins (fractionated Mw ～3000 Da, and unfractionated Mw ～17,000-19,000 Da) and examined by ESCA, AFM, and contact angle goniometer. The effect of these different surface-immobilized heparins on blood protein adsorption and on platelet adhesion, were examined after incubating the samples with platelet-poor and platelet-rich plasma. Protein adsorption kinetics was studied by electrophoresis and the platelets adhered on the surfaces were examined by SEM after incubation. The two heparin types clearly showed different behavior with respect to protein adsorption, especially in the early stages of blood plasma interaction. After a 5-min incubation in plasma, low molecular weight heparin-immobilized polyurethanes (PU-LMWH) showed three times less protein adsorption compared to unfractionated heparin-immobilized polyurethanes (PU-UFH). The total amount of adhered protein became more similar as the incubation time was extended. The morphology of adhered platelets on material surfaces demonstrated differences: PU-UFH had clusters with some pseudopodia extensions, while PU-LMWH surfaces had round-shaped platelets with little clustering. For contact times >15 min, the amount of adsorbed proteins and adhered platelets on the heparinized PU surfaces decreased.
机译：通过共价固定不同分子量的肝素（分馏的Mw〜3000 Da和未分馏的Mw〜17,000-19,000 Da）对聚氨酯表面进行改性，并用ESCA，AFM和接触角测角计进行检测。在将样品与贫血小板和富血小板血浆孵育后，检查了这些不同的表面固定化肝素对血液蛋白吸附和血小板粘附的影响。通过电泳研究蛋白质的吸附动力学，并在孵育后通过SEM检查粘附在表面上的血小板。两种肝素类型显然在蛋白质吸附方面表现出不同的行为，尤其是在血浆相互作用的早期。在血浆中孵育5分钟后，低分子量肝素固定聚氨酯（PU-LMWH）的蛋白质吸附量是普通肝素固定聚氨酯（PU-UFH）的三倍。随着孵育时间的延长，粘附蛋白的总量变得越来越相似。材料表面上粘附的血小板的形态表现出差异：PU-UFH具有簇，具有一些假足延伸，而PU-LMWH表面具有圆形的血小板，几乎没有簇。对于> 15分钟的接触时间，肝素化的PU表面吸附的蛋白质和粘附的血小板数量减少。

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