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首页> 外文期刊>Journal of biochemical and molecular toxicology >Activation of a stress-induced gene by insecticides in the midge, Chironomus yoshimatsui.
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Activation of a stress-induced gene by insecticides in the midge, Chironomus yoshimatsui.

机译：蚊虫吉田Chironomus yoshimatsui中的杀虫剂激活了应激诱导的基因。

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摘要

Stress proteins (heat shock proteins, HSPs) have been proposed as general biomarkers for environmental monitoring. In the present study, we evaluated the environmental stress-burden on the aquatic midge Chironomus yoshimatsui using hsp70 expression. Larvae collected from streams receiving polluted runoff (field strain) were resistant to the organophosphorus insecticide, fenitrothion (F), and the synthetic pyrethroid, ethofenprox (E), whereas a strain originally collected from an unpolluted area (susceptible strain) showed low resistance to insecticide exposure. To examine the expression of an HSP70 gene in C. yoshimatsui, an hsp70 cDNA probe was prepared using RNA obtained from the field strain larvae and used for Northern blot analyses. The expression of this HSP70 gene in larvae collected from two field sites in May about 1 week after insecticide spraying in the fields was 2.3 (p = 0.018) to 3.3 fold higher than that in the susceptible strain and was also 4.6 and 1.4 (p = 0.033) fold higher than those collected in November 3 months after the cessation of insecticide spraying. In order to identify potential inducers of the HSP70 gene of the field strain, larvae of the susceptible strain were exposed to F or E for 24 h and hsp70 mRNA levels determined. Exposures to F at 0.4 microg/L and E at 1.1 microg/L increased hsp70 mRNA levels 2.7 (p = 0.049) and 4.4 (p = 0.043) fold over controls, respectively. These results suggest that larvae collected from polluted areas are burdened by environmental stressors and the tested insecticides are potential inducers of HSP70. The results also support the suggestion that HSP70 gene expression is a sensitive indicator of low level (nonlethal) exposures to certain insecticides. Copyright 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:10-17, 2002; DOI 10.1002/jbt.10018
机译：应激蛋白（热激蛋白，HSPs）已被提议作为环境监测的通用生物标志物。在本研究中，我们使用hsp70表达评估了水生中蚊Yoshimatsui对环境的负担。从受到污染的径流（田间应变）的流中收集的幼虫对有机磷杀虫剂杀nitro硫磷（F）和合成的拟除虫菊酯，ethofenprox（E）具有抗性，而最初从无污染地区收集的菌株（易感株）对杀虫剂暴露。为了检验HSP70基因在吉井假丝酵母中的表达，使用从田间幼虫获得的RNA制备了hsp70 cDNA探针，并将其用于Northern印迹分析。在田间喷洒杀虫剂后约1周，5月从两个田间采集的幼虫中该HSP70基因的表达比易感菌株高2.3倍（p = 0.018）至3.3倍，分别为4.6和1.4（p =比杀虫剂喷洒停止后的11月3个月收集的收集量高0.033倍。为了鉴定田间菌株HSP70基因的潜在诱导物，将易感菌株的幼虫暴露于F或E 24小时，并测定hsp70 mRNA水平。暴露于0.4 microg / L的F和1.1 microg / L的E分别使hsp70 mRNA水平比对照组高2.7倍（p = 0.049）和4.4（p = 0.043）。这些结果表明，从受污染地区收集的幼虫会受到环境胁迫的负担，所测试的杀虫剂可能是HSP70的潜在诱因。该结果还支持HSP70基因表达是某些杀虫剂低水平（非致死性）暴露的敏感指标的建议。版权所有2002 Wiley Periodicals，Inc. J Biochem Mol Toxicol 16：10-17，2002; DOI 10.1002 / jbt.10018

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