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首页> 外文期刊>Journal of biochemical and molecular toxicology >The induction of cytochrome P450 1A1 by sudan dyes.
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The induction of cytochrome P450 1A1 by sudan dyes.

机译：苏丹染料诱导细胞色素P450 1A1。

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摘要

Azo dyes form a major class of chemically related compounds that are ubiquitous in foods, paints, printing inks, cosmetics, and also used as biological stains in histological and histopathological laboratories and clinics. Sudan I, sudan III, and sudan IV have been classified as category 3 carcinogens by International Agency for Research on Cancer. In this study, we investigated the difference between these three sudan dyes in induction of CYP1A1. We intraperitoneally treated Wistar rats with each of the three sudan dyes (I, III, and IV) for 3 days. Treatment of Wistar rats with sudan I produced the highest induction of CYP1A1 protein and mRNA whereas treatment of Wistar rats with sudan III produced about two third of CYP1A1 protein and mRNA than induced by sudan I. Furthermore, treatment of Wistar rats with sudan IV produced the lowest induction of CYP1A1 protein and mRNA which is about two third of that induced with sudan III treatment. We further investigated the effect of these sudan dyes on CYP1A1transcription through investigating the xenobiotic response element (XRE) reporter activity in HepG2. The XRE reporter activity study showed the same trend of activity of sudan dyes comparable to the effects on CYP1A1 mRNA and protein. Immunohistochemical study revealed a differential pattern of distribution of CYP1A1 protein in rat liver among the three sudan dyes, apparent in the centrilobular and midzonal region with sudan III, progressing to panlobular with sudan I, whereas sudan IV showed a reversal of pattern of induction with the most intense staining in the periportal region. Our results suggest that there is an inverse relationship between the molecular size of the three sudan dyes and their ability to induce CYP1A1.
机译：偶氮染料是一类主要的化学相关化合物，广泛存在于食品，油漆，印刷油墨，化妆品中，还用作组织学和组织病理学实验室及临床中的生物染色剂。国际癌症研究机构将苏丹I，苏丹III和苏丹IV归类为3类致癌物。在这项研究中，我们调查了这三种苏丹染料在CYP1A1诱导中的差异。我们用三种苏丹红染料（I，III和IV）分别腹膜内处理Wistar大鼠3天。用苏丹I处理Wistar大鼠产生的CYP1A1蛋白和mRNA诱导最高，而用苏丹III处理Wistar大鼠产生的CYP1A1蛋白和mRNA约是苏丹I诱导的三分之二。此外，用苏丹IV处理Wistar大鼠产生CYP1A1蛋白和mRNA的诱导。 CYP1A1蛋白和mRNA的诱导最低，约为苏丹III处理诱导的三分之二。我们通过调查HepG2中的异种应答元件（XRE）报告基因活性，进一步研究了这些苏丹染料对CYP1A1转录的影响。 XRE记者活性研究表明，苏丹染料的活性具有与对CYP1A1 mRNA和蛋白质的影响相当的趋势。免疫组织化学研究显示，CYP1A1蛋白在三种苏丹染料之间在大鼠肝脏中的分布模式不同，在苏丹III的小叶和中部区域很明显，随着苏丹I逐渐发展为全小叶，而苏丹IV则显示与苏丹I的诱导模式相反。在门周围区域最强烈的染色。我们的结果表明三种苏丹染料的分子大小与其诱导CYP1A1的能力之间存在反比关系。

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