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首页> 外文期刊>Journal of biochemistry and molecular biology >Byr4p, a Possible Regulator of Mitosis and Cytokinesis in fission Yeast, Localizes to the spindle Pole Body by its C-Terminal Domains
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Byr4p, a Possible Regulator of Mitosis and Cytokinesis in fission Yeast, Localizes to the spindle Pole Body by its C-Terminal Domains

机译：Byr4p，可能是裂变酵母中有丝分裂和胞质分裂的调节剂，通过其C末端域定位在纺锤体上

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摘要

Cytokinesis and septation should be coordinated to nuclear division in the cell division cycle for precise transmission of the genome into daughter cells. byr4, an essential gene in fission yeast Schizosaccharomyces pombe, regulates the timing of cytokinesis and septation in a dosage-dependent manner. We examined the intracellular localization of the Byr4 protein by expressing byr4 as a fusion of green fluorescence protein (GFP). The Byr4 protein localizes as a single dot on the nuclear periphery of interphase cells, duplicates before mitosis, and the duplicated dots segregate with the nuclei in anaphase. The behavior of Byr4p throughout the cell cycle strongly suggests that Byr4p is localized to the spindle pole body (SPB), a microtubule organizing center (MTOC) in yeast. The presence of the Byr4 protein in the SPB is consistent with its function to coordinate mitosis and cytokinesis. We also mapped the domains of Byr4p for its proper localization to SPB by expressing various byr4 deletion mutants as GFP fusions. Analyses of the diverse byr4 deletion mutants suggest that the indirect repeats and the regions homologous to the open reading frame (ORF) YJR53W of S. cerevisiae in its C-terminus are essential for its localization to the SPB.
机译：细胞分裂和分隔应该在细胞分裂周期中与核分裂相协调，以将基因组精确地传递到子细胞中。裂殖酵母粟酒裂殖酵母中的必需基因byr4以剂量依赖的方式调节胞质分裂和分裂的时间。我们通过将byr4表达为绿色荧光蛋白（GFP）的融合蛋白来检查Byr4蛋白的细胞内定位。 Byr4蛋白定位为间质细胞核外围的单个点，在有丝分裂之前复制，并且复制的点在后期与细胞核分离。 Byr4p在整个细胞周期中的行为强烈表明，Byr4p定位于纺锤极体（SPB），即酵母中的微管组织中心（MTOC）。 SPB中Byr4蛋白的存在与其协调有丝分裂和胞质分裂的功能一致。我们还通过将各种Byr4缺失突变体表达为GFP融合体，将Byr4p的结构域正确定位到SPB。各种byr4缺失突变体的分析表明，其C端的间接重复和与酿酒酵母开放阅读框（ORF）YJR53W同源的区域对于其定位到SPB是必不可少的。

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