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首页> 外文期刊>Journal of Biochemical and Biophysical Methods >Scientific and technological aspects of oxygen-sensitive electrodes for measurements of oxygen partial pressure in patch-clamp experiments.
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Scientific and technological aspects of oxygen-sensitive electrodes for measurements of oxygen partial pressure in patch-clamp experiments.

机译：膜片钳实验中用于测量氧气分压的氧气敏感电极的科学和技术方面。

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摘要

Anoxia/hypoxia induces dramatic changes in brain activity leading to the damages in brain structure. Several minutes of decrease in environmental oxygen partial pressure (P(O(2))) can irreversibly destroy nerve cells. Therefore, investigations of intracellular mechanisms responsible for hypoxia-induced changes of mammalian nerve system are very important. On-line adjustment and measurements of P(O(2)) in bath solution during patch-clamp experiments are especially topical. At the recent time, a special interest on the on-line measurements of oxygen contents in bath solution has appeared as a result of application of old approaches previously used for polarographic measurements of oxygen contents in the blood and tissues. Here we describe the simple method of manufacturing oxygen-sensitive microelectrodes, which can be used with standard patch-clamp amplifier. We also describe the main principles of polarographic method and properties of oxygen-sensitive electrodes used in patch-clamp experiments.
机译：缺氧/缺氧会引起大脑活动的剧烈变化，从而导致大脑结构的损害。减少几分钟的环境氧分压（P（O（2）））可以不可逆转地破坏神经细胞。因此，研究引起缺氧诱导的哺乳动物神经系统变化的细胞内机制非常重要。在膜片钳实验期间在线调整和测量浴液中的P（O（2））尤其重要。近年来，由于先前用于极谱法测量血液和组织中氧含量的旧方法的应用，人们对在线测量浴液中的氧含量表现出特别的兴趣。在这里，我们描述了制造对氧敏感的微电极的简单方法，该方法可与标准膜片钳放大器一起使用。我们还描述了极谱法的主要原理以及膜片钳实验中使用的氧敏感电极的性能。

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