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首页> 外文期刊>Journal of biochemical and molecular toxicology >The influence of diet on the regional distribution of glutathione S-transferase activity in channel catfish intestine.
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The influence of diet on the regional distribution of glutathione S-transferase activity in channel catfish intestine.

机译：饮食对channel鱼肠中谷胱甘肽S-转移酶活性区域分布的影响。

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摘要

There is evidence that glutathione conjugates are the major metabolites formed following systemic uptake of carcinogenic contaminants from the intestine. The effect of commercial diet versus a semi-purified diet on the distribution of glutathione S-transferase (GST) activity was examined in proximal, medial, and distal sections of catfish intestine. The bulk of GST activity with 1-chloro-2,4-dinitrobenzene, ethacrynic acid, and 3H-benzo[a]pyrene-4,5-oxide, and the percent cytosolic protein cross-reacting with anti-catfish GST-pi were in the more proximal segments and dropped off distally in the two diet groups. However, the total GST-pi cross-reacting protein in the proximal section was significantly higher in fish fed a chow diet. Western blot analysis revealed pi-class GST to be expressed principally in the proximal intestine. Cytosol samples cross-reacted with antibodies to human GST-alpha, -mu, and -pi, but not -theta, classes. Alpha-like GST isoforms of MW 26,200 and 24,600, absent in sections from fish fed a purified diet, were differentially expressed only in the distal section of chow-fed fish. These results indicate that diet significantly elicits regional differences in GST protein levels, that components of the commercial chow affect GST protein expression in the distal intestine, and that maintenance diet should be taken into consideration during dietary exposure studies.
机译：有证据表明，谷胱甘肽结合物是肠道中致癌污染物的系统吸收后形成的主要代谢产物。在diet鱼肠的近端，中间和远端部分检查了商业饮食与半纯饮食对谷胱甘肽S-转移酶（GST）活性分布的影响。与1-氯-2,4-二硝基苯，乙炔酸和3H-苯并[a] py-4,5-氧化物的GST活性大部分以及与抗cat鱼GST-pi交叉反应的胞质蛋白百分比在两个饮食组中，最近端的节段下降，而远端下降。但是，在喂猪饲料的鱼中，近端的总GST-pi交叉反应蛋白明显更高。蛋白质印迹分析显示pi类GST主要在近端肠中表达。细胞溶胶样品与针对人GST-alpha，-mu和-pi的抗体交叉反应，但与-theta类的抗体没有交叉反应。饲喂纯净日粮的鱼的切片中不存在分子量为26,200和24,600的Alpha样GST亚型，仅在cho鱼的末梢部分差异表达。这些结果表明，饮食显着引起GST蛋白水平的区域差异，商业食物的成分会影响远端肠中GST蛋白的表达，并且在饮食暴露研究中应考虑维持饮食。

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