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首页> 外文期刊>Journal of biochemical and molecular toxicology >Effects of siRNA Targeting c-Myc and VEGF on Human Colorectal Cancer Volo Cells
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Effects of siRNA Targeting c-Myc and VEGF on Human Colorectal Cancer Volo Cells

机译：靶向c-Myc和VEGF的siRNA对人大肠癌Volo细胞的影响

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摘要

c-Myc and vascular endothelial growth factor (VEGF) genes are frequently deregulated and overexpressed in this malignancy, and strategies designed to inhibit c-Myc and VEGF expression in cancer cells may have considerable therapeutic value. In the present study, we design and use short interfering RNA (siRNA) to inhibit c-Myc and VEGF expression in colorectal cancer Volo cells and validate their effects on cell proliferation, cell cycle, apoptosis, and cell metastasis. Upon transient transfection with plasmid-encoding siRNA, it was found that expression of c-Myc and VEGF was significantly downregulated in siRNA-transfected cells and the downregulation of c-Myc and VEGF inhibited cell growth and induced apoptosis and metastasis of Volo cells. c-Myc and VEGF downregulation also increased cell population in the G0-G1 phase. In conclusion, the specific siRNA efficiently silenced the expression of c-Myc and VEGF, further suppressed the cell proliferation, triggered cell apoptosis, and inhibited cell invasiveness of colorectal cancer Volo cells.
机译：c-Myc和血管内皮生长因子（VEGF）基因在这种恶性肿瘤中经常失调和过度表达，设计用于抑制癌细胞中c-Myc和VEGF表达的策略可能具有相当的治疗价值。在本研究中，我们设计并使用短干扰RNA（siRNA）抑制结直肠癌Volo细胞中c-Myc和VEGF的表达，并验证其对细胞增殖，细胞周期，细胞凋亡和细胞转移的影响。用编码siRNA的质粒瞬时转染后，发现siRNA转染的细胞中c-Myc和VEGF的表达显着下调，而c-Myc和VEGF的下调抑制了细胞的生长，并诱导了Volo细胞的凋亡和转移。 c-Myc和VEGF的下调也增加了G0-G1期的细胞数量。总之，特异性siRNA有效沉默了c-Myc和VEGF的表达，进一步抑制了细胞增殖，触发了细胞凋亡，并抑制了结直肠癌Volo细胞的侵袭性。

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