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首页> 外文期刊>Journal of biochemical and molecular toxicology >Chemopreventive Properties of Genipin on AGS Cell Line via Induction of JNK/Nrf2/ARE Signaling Pathway
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Chemopreventive Properties of Genipin on AGS Cell Line via Induction of JNK/Nrf2/ARE Signaling Pathway

机译：Genipin通过诱导JNK / Nrf2 / ARE信号通路对AGS细胞系的化学预防性质

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Roles of dietary phytochemicals in cancer chemoprevention via induction of nuclear factor-erythroid-2-related factor 2 (Nrf2)-mediated antioxidant enzymes have been well established in a number of studies. In this study, FACS analysis was used to reveal that the intracellular reactive oxygen species level decreased at 0-25 M of genipin treatment. Furthermore, immunofluorescence analysis and Western blotting were used to demonstrate that genipin treatment resulted in the upregulation and nuclear translocation of Nrf2, as well as upregulation of gastrointestinal glutathione peroxidase. Finally, we found that C-Jun-NH2-kinase (JNK) was also dose-dependently activated, where depleting JNK by using a biochemical inhibitor indicated that JNK was upstream of Nrf2. Interestingly, the antioxidant effects were limited to the treatment in the lower dosage of genipin, where higher dosage of genipin treatment resulted in the increased reactive oxygen species level and cytotoxicity. Thus, this study demonstrates for the first time that lower dosage of genipin results in the induction of JNK/Nrf2/ARE signaling pathway and protection from cell death.
机译：饮食中的植物化学物质通过诱导核因子-类胡萝卜素-2相关因子2（Nrf2）介导的抗氧化酶在癌症化学预防中的作用已在许多研究中确立。在这项研究中，流式细胞仪分析被用来揭示细胞内活性氧水平在京尼平处理0-25 M时降低。此外，免疫荧光分析和Western印迹被用来证明genipin处理导致Nrf2的上调和核转位，以及胃肠道谷胱甘肽过氧化物酶的上调。最后，我们发现C-Jun-NH2-激酶（JNK）也被剂量依赖性激活，其中通过使用生化抑制剂耗尽JNK表示JNK在Nrf2的上游。有趣的是，抗氧化剂的作用仅限于较低剂量的Genipin的治疗，其中较高剂量的Genipin处理导致增加的活性氧水平和细胞毒性。因此，这项研究首次证明了较低的Genipin剂量可诱导JNK / Nrf2 / ARE信号通路并保护细胞免受死亡。

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