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首页> 外文期刊>Journal of biochemical and molecular toxicology >The action of zymosan on octanoate transport and metabolism in the isolated perfused rat liver.
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The action of zymosan on octanoate transport and metabolism in the isolated perfused rat liver.

机译：酵母聚糖对离体灌流大鼠肝脏中辛酸转运和代谢的作用。

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摘要

The effects of zymosan on transport, distribution, and metabolism of octanoate in the perfused rat liver were investigated using the multiple-indicator dilution technique. Livers were perfused with 300 microM octanoate in the absence or in the presence of 100 microg/mL zymosan. Tracer amounts of [1-14C]octanoate, [3H] water, and [131I]albumin were injected into the portal vein, and the effluent perfusate was fractionated. The normalized dilution curves were analyzed by means of a space-distributed variable transit time model. Zymosan decreased the space into which octanoate undergoes flow-limited distribution, possibly the first cellular exchanging pool represented by plasma membranes and their adjacencies. However, the rate of transfer of octanoate from the plasma membrane into the rest of the cell was not modified as indicated by the similar values of the influx rates and also the net uptake of octanoate per unit of accessible cellular volume. However, when referred to the wet weight of the liver, the net uptake of octanoate was 37.5% reduced, a value corresponding to the diminution of the cellular accessible space. It can be concluded that an exclusion of a fraction of the liver parenchyma from the microcirculation is the main mechanism by which zymosan reduces the metabolism of exogenous octanoate.
机译：使用多指标稀释技术研究了酵母聚糖对灌注大鼠肝脏中辛酸的转运，分布和代谢的影响。在不存在或存在100 microg / mL酵母聚糖的情况下，向肝脏灌注300 microM辛酸酯。将示踪量的[1-14C]辛酸酯，[3H]水和[131I]白蛋白注入门静脉，并对流出的灌注液进行分级分离。通过空间分布的可变渡越时间模型分析归一化的稀释曲线。酵母聚糖减少了辛酸酯进入限流分布的空间，这可能是由质膜及其邻接物代表的第一个细胞交换池。然而，辛酸从质膜转移到细胞其余部分的速率没有改变，这是由流入速率的相似值以及每单位可及细胞体积的辛酸酯净吸收值所表明的。但是，以肝脏的湿重计，辛酸的净摄取减少了37.5％，该值对应于细胞可及空间的减少。可以得出结论，从微循环中排除一部分肝实质是酵母聚糖降低外源性辛酸酯代谢的主要机制。

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