• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biochemistry and molecular biology >Cytotoxicity of Vibrio Vulnificus Cytolysin on Rat Neutrophils
【24h】

Cytotoxicity of Vibrio Vulnificus Cytolysin on Rat Neutrophils

机译：创伤弧菌溶细胞素对大鼠中性粒细胞的细胞毒性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cytolysin produced by Vibrio vulnificus has been known to be lethal to mice by increasing vascular permeability and neutrophil sequestration in the lung. In the present study, a cytotoxic mechanism of V. vulnificus cytolysin on the neutrophil was investigated. Cytolysin rapidly bound to neutrophils and induced cell death, as determined by the trypan blue exclusion test. V. vulnificus cytolysin caused the depletion of cellular ATP without the release of ATP or lactate dehydrogenase. Formation of transmembrane pores was evidenced by the rapid efflux of potassium and 2-deoxy-d-[~3H]glucose from cytolysin-treated neutrophils. It was further confirmed by the rapid flow of monovalent ions in the patch clamp of cytolysin-treated neutrophil membrane. The pore formation was accompanied by the oligomerization of cytolysin monomers on the neutrophil membrane as demonstrated by immunoblot, which exhibited a 210 kDa band corresponding to a tetramer of the native cytolysin of M_r 51,000. These findings indicate that V. vulnificus cytolysin rapidly binds to the neutrophil membrane and oligomerizes to form small transmembrane pores, which induce the efflux of potassium and the depletion of cellular ATP leading to cell death without cytolysis.
机译：已知由创伤弧菌产生的细胞溶素可通过增加血管的通透性和肺中性粒细胞的隔离而对小鼠致死。在本研究中，研究了创伤弧菌溶血素对嗜中性粒细胞的细胞毒性机制。胞溶素迅速结合中性粒细胞并诱导细胞死亡，这是通过台盼蓝排除试验确定的。 V. vulnificus溶细胞素导致细胞ATP耗竭，而没有释放ATP或乳酸脱氢酶。钾和2-脱氧-d- [〜3H]葡萄糖从溶血素处理过的中性粒细胞中快速流出证明了跨膜孔的形成。单价离子在溶细胞素处理的嗜中性粒细胞膜的膜片钳中快速流动进一步证实了这一点。如免疫印迹所证实，孔的形成伴随着嗜中性粒细胞膜上的溶细胞素单体的低聚，其表现出210kDa的条带，其对应于M_r 51,000的天然溶细胞素的四聚体。这些发现表明，创伤弧菌的溶血素快速结合中性粒细胞膜并寡聚形成小的跨膜孔，其引起钾的流出和细胞ATP的消耗，导致细胞死亡而无细胞溶解作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号