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首页> 外文期刊>Journal of biomaterials applications >Ectopic bone formation in adipose-derived stromal cell-seeded osteoinductive calcium phosphate scaffolds.
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Ectopic bone formation in adipose-derived stromal cell-seeded osteoinductive calcium phosphate scaffolds.

机译：脂肪来源的基质细胞接种的骨诱导性磷酸钙支架中的异位骨形成。

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摘要

The phenomenon of osteoinduction by biomaterials has been proven and used in animals. However, whether the ability of a biomaterial to initiate bone formation in ectopic implantation sites improves the performance of such osteoinductive biomaterial as a scaffold for tissue-engineered (TE) bone remains unclear. In this study, we compared ectopic bone formation by combining autologous adipose-derived stromal cells (ADSCs) with an osteoinductive and a nonosteoinductive biphasic calcium phosphate (BCP) ceramic to create a tissue engineering construct in the muscle of dogs. Two groups of BCP scaffolds (BCP1 and BCP2) were prepared. In each group, ADSCs were seeded, and the scaffolds without seeded cells served as controls. All implants were implanted in the back muscle of 10 adult dogs for 8 weeks and 12 weeks. Microcomputed tomography (Micro-CT) analysis and histomorphometry were performed to evaluate and quantify ectopic bone formation. The results indicated that the osteoinductive BCP1 performed significantly better compared to the nonosteoinductive BCP2 in cell-based TE bone formation ectopically. The ADSCs had a significantly positive effect on the ectopic bone formation. In addition, the usefulness of Micro-CT for the efficient and nondestructive analysis of mineralized bone and calcium phosphate scaffold was confirmed.
机译：已经证明了通过生物材料引起的骨诱导现象并已在动物中使用。然而，生物材料在异位植入部位引发骨形成的能力是否能改善这种骨诱导性生物材料作为组织工程（TE）骨支架的性能尚不清楚。在这项研究中，我们通过将自体脂肪来源的基质细胞（ADSC）与骨诱导性和非骨诱导性双相磷酸钙（BCP）陶瓷相结合，在狗的肌肉中创建了组织工程构造，比较了异位骨的形成。准备了两组BCP支架（BCP1和BCP2）。在每组中，接种ADSC，并且将没有接种细胞的支架用作对照。将所有植入物植入10只成年犬的背部肌肉中，分别植入8周和12周。进行了微计算机断层扫描（Micro-CT）分析和组织形态计量学，以评估和量化异位骨形成。结果表明在异位基于细胞的TE骨形成中，骨诱导性BCP1比非骨诱导性BCP2表现更好。 ADSC对异位骨形成具有明显的积极影响。另外，证实了Micro-CT对于矿化的骨和磷酸钙支架的有效和非破坏性分析的有用性。

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