Production and Characterization of Active Soluble Human #beta#1,4-Galactosyltransferase in Escherichia coli as a Useful Catalyst in Synthesis of the Gal #beta#beta#(->)4 GlcNAc Linkage

Shigeo Shibatani; Kazuhito Fujiyama; Susumu Nishiguchi; Tatsuji Seki; Yoshihiko Maekawa

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Production and Characterization of Active Soluble Human #beta#1,4-Galactosyltransferase in Escherichia coli as a Useful Catalyst in Synthesis of the Gal #beta#beta#(->)4 GlcNAc Linkage

机译：活性可溶性人＃beta＃1,4-半乳糖基转移酶在大肠杆菌中的生产和表征，可作为合成Gal＃beta＃beta＃（->）4 GlcNAc连锁反应的有用催化剂

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An active and sluble human #beta#1,4-galactosyltransferase(#beta#-GT) was produced in Escherichia coli using a maltose-binding protein fusion system.The purified recombiant #beta#-GT has a K_m value of 0.035mM for UDP-galactose and a V_(max) of 643X10~3nmol/mg/h.The enzyme catalyzes the transfer of galactose from UDP-galactose to N-linked oligosaccharides.The properties of the purified enzyme were identical to those of bovine milk #beta#-GT.

机译：使用麦芽糖结合蛋白融合系统在大肠杆菌中生产了一种活性且可溶性的人＃beta＃1,4-半乳糖基转移酶（＃beta＃-GT）。纯化的重组蛋白＃beta＃-GT的K_m值为0.035mM UDP-半乳糖和V_（max）为643X10〜3nmol / mg / h。该酶催化将半乳糖从UDP-半乳糖转移至N-连接的寡糖，纯化后的酶的性质与#beta牛乳相同＃-GT

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来源
《Journal of Bioscience and Bioengineering》 |2001年第1期|共3页
作者
Shigeo Shibatani; Kazuhito Fujiyama; Susumu Nishiguchi; Tatsuji Seki; Yoshihiko Maekawa;
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正文语种 eng
中图分类生物化学;生物工程学（生物技术）;酿造工业;
关键词
escherichia coli; btea; 1; 4-galactosyltransferase; maltose-binding protein;

机译：大肠杆菌;btea;1;4-半乳糖基转移酶;麦芽糖结合蛋白;

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1. Production and Characterization of Active Soluble Human #beta#1,4-Galactosyltransferase in Escherichia coli as a Useful Catalyst in Synthesis of the Gal #beta#beta#(->)4 GlcNAc Linkage [J] . Shigeo Shibatani, Kazuhito Fujiyama, Susumu Nishiguchi, Journal of Bioscience and Bioengineering . 2001,第1期

机译：活性可溶性人＃beta＃1,4-半乳糖基转移酶在大肠杆菌中的生产和表征，可作为合成Gal＃beta＃beta＃（->）4 GlcNAc连锁反应的有用催化剂
2. An Engineered Biocatalyst for the Synthesis of Glycoconjugates: Utilization of #beta#1,3-N-Acetyl-D-glucosaminyltransferasefrom Streptococcus agalactiade Type Ia Expressed in Escherichia coli as a Fusion with Maltose-Binding Trotein [J] . Atsushi Toda, Kuriko Yamada, Shin-Ichiro Nishimura Advanced synthesis & catalysis . 2002,第1期

机译：合成糖缀合物的工程化生物催化剂：利用表达于大肠杆菌中的Ia型无乳链球菌＃beta＃1,3-N-乙酰-D-氨基葡萄糖基转移酶作为与麦芽糖结合肌钙蛋白的融合体而利用
3. Human ovarian cancer,lymphoma spleen,and bovine milk GlcNAc:#beta#1,4Gal/GalNAc transferases:two molecular species in ovarian tumor and induction of GalNAc#beta#1,4Glc synthesis by #alpha#-lactalbumin [J] . E.V.Chandrasekaran Carbohydrate research . 2001,第2期

机译：人卵巢癌，淋巴瘤脾和牛乳GlcNAc：＃beta＃1,4Gal / GalNAc转移酶：卵巢肿瘤中的两种分子种类和＃alpha＃-乳清蛋白诱导GalNAc＃beta＃1、4Glc的合成
4. A NEW MONOCLONAL ANTIBODY RECOGNIZING THE FULL-LENGTH INTRACELLULAR HUMAN A1,4-GALACTOSYLTRANSFERASE (GB3/CD77 SYNTHASE) AS WELL AS ITS SOLUBLE FORM IN THE BLOOD SERUM [C] . Katarzyna Szymczak-Kulus, Krzysztof Mikolajczyk, Anna Bereznicka International Carbohydrate Symposium . 2018

机译：一种新的单克隆抗体，识别全长细胞内人A1,4-半乳糖基转移酶（GB3 / CD77合成酶）以及其血清中可溶形式
5. Effects of soluble polylactic acid and gamma irradiation on ground beef inoculated with Escherichia coli 0157:H7 and legal classification of irradiation as a food additive. [D] . Wilson, Thomas P. 1998

机译：可溶性聚乳酸和γ射线辐照对接种大肠杆菌0157：H7的碎牛肉的影响以及辐照作为食品添加剂的合法分类。
6. P1 Trisaccharide (Galα14Galβ14GlcNAc) Synthesis by Enzyme Glycosylation Reactions Using Recombinant Escherichia coli [O] . Ziye Liu, Yuquan Lu, Jianbo Zhang, 2003

机译：重组大肠杆菌酶糖基化反应合成P1三糖（Galα14Galβ14GlcNAc）
7. Biochemical Characterization of UDP-Gal:GlcNAc-Pyrophosphate-Lipid β-1,4-Galactosyltransferase WfeD, a New Enzyme from Shigella boydii Type 14 That Catalyzes the Second Step in O-Antigen Repeating-Unit Synthesis▿ † [O] . Xu, Changchang, Liu, Bin, Hu, Bo, 2010

机译：UDP-Gal：GlcNAc-焦磷酸盐-脂质β-1,4-半乳糖基转移酶WfeD的生化特性，这是一种来自博氏志贺氏菌的新型酶，可催化O抗原重复单元合成的第二步▿†

Production and Characterization of Active Soluble Human #beta#1,4-Galactosyltransferase in Escherichia coli as a Useful Catalyst in Synthesis of the Gal #beta#beta#(->)4 GlcNAc Linkage

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