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首页> 外文期刊>Journal of Bioscience and Bioengineering >A hapl Mutation in a Laboratory Strain of Saccharomyces cerevisiae Results in Decreased Expression of Ergosterol-Related Genes and Cellular Ergosterol Content Compared to Sake Yeast
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A hapl Mutation in a Laboratory Strain of Saccharomyces cerevisiae Results in Decreased Expression of Ergosterol-Related Genes and Cellular Ergosterol Content Compared to Sake Yeast

机译：与酿酒酵母相比，酿酒酵母实验室菌株中的半乳糖突变导致麦角固醇相关基因的表达减少和细胞中麦角固醇的含量降低

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摘要

DNA microarray and Northern blot analysis revealed that a sake yeast strain Kyokai no.7 (K7) showed higher expression of genes encoding proteins involved in ergosterol biosynthesis than a laboratory yeast strain X2180.We hypothesized that these differences in expression levels were caused by a defect of a transcriptional factor Hapl,because strain X2180 contained a Tyl insertion mutation in the HAP1 gene.To confirm this,we constructed a strain X2180 derivative (strain HX) that contained the wild-type HAP1 genes originating from strain K7.The expression levels of ergosterol-related genes and cellular ergosterol content in strain HX were higher than those in strain X2180 and were almost comparable to those in strain K7.These results suggest that the differences in the expression levels of ergosterol-related genes and ergosterol content between strains K7 and X2180 were largely caused by the hapl mutation in strain X2180.Involvement of the mutated Hapl in the differential gene expression between strain K7 and strain X2180 was further confirmed by a lacZ reporter assay of HMG1,one of the Hapl-regulated genes.We also revealed that the HMG1 promoter region between-500 and-376 was important in the transcriptional activation by Hapl.
机译：DNA芯片和Northern印迹分析显示，清酒酵母菌株Kyokai No.7（K7）与实验室酵母菌株X2180相比，显示出参与麦角固醇生物合成的蛋白质编码基因的表达更高。我们假设这些表达水平的差异是由缺陷引起的。转录因子Hapl的表达，因为X2180菌株在HAP1基因中含有一个Tyl插入突变。为证实这一点，我们构建了一个X2180衍生物（HX菌株），其中包含源自K7菌株的野生型HAP1基因。 HX菌株中的麦角固醇相关基因和细胞中的麦角甾醇含量均高于X2180菌株，与K7菌株中的麦角固醇相关基因和细胞中麦角固醇含量几乎相同。 X2180主要由X2180菌株中的hapl突变引起.Hapl突变与差异基因表达有关。通过Hap1调控基因之一HMG1的lacZ报告基因分析进一步证实了K7菌株和X2180菌株之间的同源性。我们还揭示了-500和-376之间的HMG1启动子区域在Hapl的转录激活中很重要。

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