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首页> 外文期刊>Journal of Bioscience and Bioengineering >Drastic change in cell surface hydrophobicity of a new bacterial strain, Pseudomonas sp. TIS1-127, induced by growth temperature and its effects on the toluene-conversion rate
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Drastic change in cell surface hydrophobicity of a new bacterial strain, Pseudomonas sp. TIS1-127, induced by growth temperature and its effects on the toluene-conversion rate

机译：新细菌菌株假单胞菌菌种细胞表面疏水性的急剧变化。 TIS1-127，受生长温度的影响及其对甲苯转化率的影响

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In a previous study, we reported the effectiveness of a bacterial strain showing monolayer adsorption to oil surfaces on microbial conversion at oil-water interfaces. In the present study, we screened wild type strains from our toluene-degrading bacterial library that showed similar properties and succeeded in obtaining five wild type strains that adsorb to oil surfaces as a cell monolayer. We investigated the effects of cultivation conditions on cell surface hydrophobicity of these five strains. The effects of substrate hydrophobicity and the porous carrier were not significant By contrast, growth temperature greatly affected the cell surface hydrophobicity of all five strains, especially strain TIS1-127, which was phylogenetically identified as Pseudomonas sp. which is closely related to P. mosselii, P. monteilii, and P. plecoglossicida. Pseudomonas sp. TIS1-127 cells grown at 37 °C were determined by the kinetic microbial-adhesion-to-hydrocarbon (MATH) test to be fully hydrophilic (lower than 10% of MATH value) while the cells grown at 28 °C were highly hydrophobic (over 90% of MATH value). We investigated the effects of growth temperature on toluene conversion by TBI-127 resting cells in single-phase batch cultivation and in two-liquid-phase partitioning reactors containing an emulsion consisting of 20% silicone oil and 80% cell suspension. In both cases, the cells grown at 28 °C showed much higher conversion ability than those grown at 37 °C. Toluene conversion followed Michaelis-Menten kinetics and the K_m values for the cells grown at 28 °C were lower than 1/10 those for the cells grown at 37 °C.
机译：在先前的研究中，我们报道了一种细菌菌株的有效性，该菌株在油水界面的微生物转化过程中表现出单层吸附至油表面的能力。在本研究中，我们从甲苯降解细菌文库中筛选了具有相似特性的野生型菌株，并成功获得了五种野生型菌株，它们作为细胞单层吸附在油表面。我们调查了培养条件对这五个菌株的细胞表面疏水性的影响。相比之下，底物疏水性和多孔载体的影响并不显着。相反，生长温度极大地影响了所有五个菌株的细胞表面疏水性，特别是系统发育上被鉴定为假单胞菌属的菌株TIS1-127。与P. mosselii，P。monteilii和P. plecoglossicida密切相关。假单胞菌通过动力学微生物粘附至碳氢化合物（MATH）测试确定在37°C下生长的TIS1-127细胞具有完全亲水性（低于MATH值的10％），而在28°C下生长的细胞具有高度疏水性（超过数学值的90％）。我们调查了TBI-127静止细胞在单相分批培养中以及在包含由20％硅油和80％细胞悬浮液组成的乳液的两液相分配反应器中，生长温度对甲苯转化的影响。在这两种情况下，在28°C下生长的细胞都比在37°C下生长的细胞具有更高的转化能力。甲苯转化遵循Michaelis-Menten动力学，在28°C下生长的细胞的K_m值低于在37°C下生长的细胞的K_m值。

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