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首页> 外文期刊>Journal of Biotechnology >Evaluation of spent medium recycle and nutrient feeding strategies forrecombinant protein production in the insect cell-baculovirus process
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Evaluation of spent medium recycle and nutrient feeding strategies forrecombinant protein production in the insect cell-baculovirus process

机译：评估昆虫细胞-杆状病毒过程中重组蛋白生产的废培养基循环和养分补料策略

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摘要

In the production of recombinant proteins by the insect cell-baculovirus process, fresh medium renewal prior to virus infection has been an effective measure for maintaining high protein yields. The spent medium used in the growth stage may still be unexhausted and can be reused for cell growth. In this study, we exercised the recycle of a spent insect cell medium for cell growth and recombinant protein production. It was found that the spent medium collected from a culture close to the stationary growth phase could provide full support for insect cell growth through another batch culture after fortified with suitable nutrients (yeastolate, glucose and glutamine) and a small fraction (15-20%) of fresh medium. The specific and volumetric protein yields with cells grown in the recycled spent medium were in most cases similar to those with cells grown in fresh medium. Protein yields on per unit volume of fresh medium in the process incorporating spent medium recycle compared favourably with that achieved in a batch or fed-batch process. Spent medium recycle is hence a feasible alternative for the insect cell culture-baculovirus process.
机译：在通过昆虫细胞-杆状病毒过程生产重组蛋白时，在病毒感染之前更新新鲜培养基一直是保持高蛋白产量的有效措施。在生长阶段使用的废培养基可能仍未耗尽，可以重新用于细胞生长。在这项研究中，我们对废昆虫细胞培养基进行了回收，以用于细胞生长和重组蛋白生产。研究发现，在添加了适当的营养素（大豆油酸酯，葡萄糖和谷氨酰胺）和一小部分（15-20％）强化后，从接近静止生长期的培养物中收集到的废培养基可以通过另一批培养为昆虫细胞的生长提供完全支持。 ）的新鲜培养基。在回收的废培养基中生长的细胞的比重和体积蛋白质产量在大多数情况下与在新鲜培养基中生长的细胞相似。与分批或补料分批工艺相比，在结合废培养基回收的过程中，每单位新鲜培养基的蛋白质产量具有优势。因此，用过的培养基循环是昆虫细胞培养-杆状病毒过程的可行替代方法。

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