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首页> 外文期刊>Journal of Biotechnology >Lactic acid production by immobilized Lactobacillus casei in recycle batch reactor: a step towards optimization
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Lactic acid production by immobilized Lactobacillus casei in recycle batch reactor: a step towards optimization

机译：在循环间歇反应器中固定化干酪乳杆菌生产乳酸：迈向优化的一步

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摘要

Nutritional and process parameters that influenced lactic acid production by Lactobacillus casei adsorbed onto Poraver beads in a recycle batch reactor system, were investigated. The presence of lactose, even as a minor proportion of total sugars, was necessary for complete utilization for growth and conversion to lactate. Hydrolysed whey protein was a richer source of nitrogen than was yeast extract. Addition of lactate to the medium at the start of the process caused severe inhibition. Optimal pHfor the fermentation process was 6.0, and the overall productivity of the recycle system under all conditions studied was greater than that obtained with a batch process using free cells. Enhancement of lactic acid production in the recycle process wasaccompanied by an increase in density of suspended cells, but the number of suspended cells did not affect overall productivity of the reactor. Bead size of the matrix was found to be important for operational stability of the reactor.
机译：研究了在循环间歇反应器系统中影响干酪乳杆菌吸附到Poraver磁珠上的乳酸生产的营养和工艺参数。乳糖的存在，甚至占糖总量的一小部分，对于完全利用生长和转化为乳酸是必需的。水解乳清蛋白比酵母提取物富含氮。在过程开始时向培养基中添加乳酸会引起严重的抑制作用。发酵过程的最佳pH为6.0，在所有研究的条件下，循环系统的总生产率均高于使用游离细胞的分批过程所获得的生产率。循环过程中乳酸产量的增加伴随着悬浮细胞密度的增加，但是悬浮细胞的数量并不影响反应器的整体生产率。发现基质的珠粒尺寸对于反应器的操作稳定性是重要的。

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