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首页> 外文期刊>Journal of Biotechnology >Purification of phycobiliproteins from Nostoc sp. by aminohexyl-Sepharosechromatography
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Purification of phycobiliproteins from Nostoc sp. by aminohexyl-Sepharosechromatography

机译:从Nostoc sp。中纯化藻胆蛋白。氨基己基-琼脂糖色谱法

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摘要

Nostoc sp. crude extract was treated with rivanol and then applied to a column of aminohexyl-Sepharose where C-phycoerythrin-II- and C-phycocyanin-containing fractions were eluted with 9.0 mM and 0.1 M phosphate buffer (pH 7.2), respectively. Both fractions were precipitated with 2.4 M ammonium sulfate. Pure C-phycoerythrin-II and free of other phycobiliproteins C-phycocyanin were obtained.
机译:Nostoc sp。粗提取物用利凡诺醇处理,然后加到氨基己基-琼脂糖柱上,分别用9.0 mM和0.1 M磷酸盐缓冲液(pH 7.2)洗脱含C-藻红蛋白II和C藻蓝蛋白的级分。两种级分均用2.4 M硫酸铵沉淀。获得纯的C-藻红蛋白-II,不含其他藻胆蛋白C-藻蓝蛋白。

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