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首页> 外文期刊>Journal of Biotechnology >Silk gland sericin protein membranes: Fabrication and characterization for potential biotechnological applications
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Silk gland sericin protein membranes: Fabrication and characterization for potential biotechnological applications

机译：丝腺丝胶蛋白膜：潜在生物技术应用的制备和表征

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摘要

This study describes the potential use of silk gland sericin protein as a biocompatible natural biopolymer in its native form,The membranes were fabricated using native silk sericin protein extracted from middle silk gland of Antheraea mylitta, a non-mulberry tropical tasar silkworm without using any cross-linking agent. The fabricated membranes were biophysically characterized and optimized for cell culture. Silk sericin protein extracted from gland contained higher amount of beta-sheets, which increased upon treatment with ethanol as observed by MR and XRD. The membranes did show robustness, good mechanical strength and high temperature stability. Cytocompatibility of the membranes was evaluated by MTT assay and cell cycle analysis using feline fibroblast cells. Morphology of growing cells was assessed by confocal microscopy that indicated normal spreading and proliferation on the silk sericin membranes. The membranes showed low inflammatory response as observed assaying TNF alpha release. This study reveals the potential of native silk sericin protein from silk gland as biocompatible biopolymer for potential biomedical applications.
机译：这项研究描述了蚕丝丝胶蛋白作为天然形式的生物相容性天然生物聚合物的潜在用途，该膜是使用从An蚕非tropical桑sar蚕的中间蚕丝丝中提取的天然蚕丝丝蛋白制备的，无需使用任何杂交-链接剂。对制成的膜进行了生物物理表征，并针对细胞培养进行了优化。 MR和XRD观察到，从腺体中提取的丝丝胶蛋白含有更高含量的β-折叠，其在乙醇处理后增加。膜确实显示出坚固性，良好的机械强度和高温稳定性。通过MTT测定和使用猫成纤维细胞的细胞周期分析来评价膜的细胞相容性。通过共聚焦显微镜评估生长中细胞的形态，表明在丝胶蛋白膜上正常扩散和增殖。如测定TNFα释放所观察到的，膜显示出低炎症反应。这项研究揭示了来自丝腺的天然丝胶蛋白作为潜在生物医学应用的生物相容性生物聚合物的潜力。

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