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首页> 外文期刊>Journal of Biotechnology >Alginate encapsulation technology supports embryonic stem cells differentiation into insulin-producing cells
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Alginate encapsulation technology supports embryonic stem cells differentiation into insulin-producing cells

机译：海藻酸盐封装技术支持胚胎干细胞分化为产生胰岛素的细胞

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摘要

This work investigates an application of the alginate encapsulation technology to the differentiation of embryonic stem (ES) cells into insulin-producing cells. It shows that the ES cells an efficiently be encapsulated within the alginate beads, retaining a high level of cell viability. The alginate encapsulation achieves approximately 10-fold increase in the cell density in the culture, in comparison to the two-dimensional conditions, opening a potential benefit of the technology in large-scale cell culture applications. Manipulations of encapsulation conditions, particularly of the initial alginate concentration, allow the control overboth the diffusion of molecules into the alginate matrix (e.g. differentiation factors) as well as control over the matrix porosity/flexibility to permit the proliferation and growth of encapsulated ES aggregates within the bead. Post-differentiation analysis confirms the presence of insulin-positive cells, as judged from immunostaining, insulin ELISA and RT-PCR analysis. The functionality of the encapsulated and differentiated cells was confirmed by their insulin production capability, whereby on glucose challenge the insulin production by the cells differentiated within alginate beads was found to be statistically significantly higher than for the cells from conventional two-dimensional differentiation system.
机译：这项工作研究了藻酸盐封装技术在将胚胎干（ES）细胞分化为产生胰岛素的细胞中的应用。它表明ES细胞被有效地封装在藻酸盐珠粒内，保持了高水平的细胞活力。与二维条件相比，藻酸盐包封使培养物中的细胞密度提高了约10倍，为大规模细胞培养应用带来了该技术的潜在优势。封装条件的控制，特别是初始藻酸盐浓度的控制，允许控制分子扩散到藻酸盐基质中（例如分化因子），以及控制基质的孔隙率/柔韧性，以允许封装的ES聚集体在其中扩散和生长珠子。通过免疫染色，胰岛素ELISA和RT-PCR分析判断，分化后的分析证实了胰岛素阳性细胞的存在。胶囊化和分化的细胞的功能性通过其胰岛素产生能力得以证实，由此在葡萄糖激发下，发现藻酸盐珠内分化的细胞的胰岛素产生在统计学上显着高于常规二维分化系统的细胞。

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