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首页> 外文期刊>Journal of Biotechnology >Expression of Hemagglutinin-Neuraminidase glycoprotein of Newcastle Disease Virus in agroinfiltrated Nicotiana benthamiana plants
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Expression of Hemagglutinin-Neuraminidase glycoprotein of Newcastle Disease Virus in agroinfiltrated Nicotiana benthamiana plants

机译：农杆菌渗入的本氏烟草植物中新城疫病毒血凝素-神经氨酸酶糖蛋白的表达

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摘要

The worldwide need for producing safer and less expensive vaccines with minor manufacture and processing requirements, together with the advances made through biotechnology, has promoted the development of efficient alternative tools to conventional vaccines. One of these is the use of plants or plant cell culture as production platforms of vaccine antigens with potential use as immunogens. We have already described the use of transgenic potato plants as immunogens against Newcastle Disease Virus (NDV), although the amount of the recombinant antigen recovered was low. The main objective of the work presented here was to enhance the expression of the HN glycoprotein of NDV through a protein targeting strategy and a promoter change. We have cloned the HN coding region under the regulation of the rubisco small subunit promoter in 5 different versions in a subcellular localization strategy, and we have established that the construct harboring the complete HN gene with its own signal peptide, fused to KDEL retention peptide, rendered the best expressed/accumulated HN protein level whether a transient or a stable transformation assay was performed. We conclude that agroinfiltration results in a simple and useful tool for selecting suitable genetic constructions to be used in stable plant transformation and, moreover, it could be used as a method to produce immunogens for vaccine developments.
机译：全球范围内对生产安全性要求较低且生产和加工要求较低的疫苗的需求，以及通过生物技术取得的进步，促进了常规疫苗高效替代工具的开发。其中之一是将植物或植物细胞培养物用作疫苗抗原的生产平台，并有可能用作免疫原。我们已经描述了使用转基因马铃薯植物作为针对新城疫病毒（NDV）的免疫原，尽管回收的重组抗原数量很少。此处提出的工作的主要目的是通过蛋白质靶向策略和启动子改变来增强NDV的HN糖蛋白的表达。我们已在rubisco小亚基启动子的调控下以亚细胞定位策略在5个不同版本中克隆了HN编码区，并且我们已经确定该构建体包含带有其自身信号肽的完整HN基因，并与KDEL保留肽融合，无论进行瞬时还是稳定的转化分析，均能获得最佳的表达/累积HN蛋白水平。我们得出的结论是，农杆菌渗入产生了一个简单而有用的工具，可以选择合适的遗传结构用于稳定的植物转化，此外，它还可以用作生产用于疫苗开发的免疫原的方法。

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