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首页> 外文期刊>Journal of Biotechnology >Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics
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Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics

机译：比较代谢组学揭示了酿酒酵母代谢和应激反应的接种量依赖性相互作用调节

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摘要

To investigate the metabolic regulation against inoculum density and stress response to high cell density, comparative metabolomic analysis was employed on Saccharomyces cerevisiae under fermentations with five different inoculum sizes by gas chromatography time-of-flight mass spectrometry. Samples from these fermentations were clearly distinguished by principal components analysis, indicating that inoculum size had a profound effect on the metabolism of S. cerevisiae. Potential biomarkers responsible for the discrimination were identified as glycerol, phosphoric acid, succinate, glycine, isoleucine, proline, palmitoleic acid, myo-inositol and ethanolamine. It indicated that enhanced stress protectants in glycerol biosynthesis and amino acid metabolism, depressed citric acid cycle intermediates, as well as decreased metabolites relating to membrane structure and function were involved as the inoculum size of yeast increased. Furthermore, significantly higher levels of glycerol and proline in yeast cells of higher inoculum size fermentation (40glp#) revealed that they played important roles in protecting yeast from stresses in high cell density fermentation. These findings provided new insights into characterizing the metabolic regulation and stress response depending on inoculum density during ethanol fermentation.
机译：为了研究针对接种物密度和对高细胞密度的胁迫响应的代谢调控，通过气相色谱飞行时间质谱对五个不同接种物发酵条件下的酿酒酵母进行了比较代谢组学分析。通过主成分分析可以清楚地区分来自这些发酵的样品，这表明接种量对酿酒酵母的代谢具有深远的影响。鉴别出可能造成歧视的生物标志物是甘油，磷酸，琥珀酸，甘氨酸，异亮氨酸，脯氨酸，棕榈油酸，肌醇和乙醇胺。研究表明，随着酵母菌接种量的增加，甘油生物合成和氨基酸代谢中增强的应激保护剂，柠檬酸循环中间体的降低以及与膜结构和功能有关的代谢产物的减少也参与其中。此外，较高接种量发酵（40glp＃）的酵母细胞中甘油和脯氨酸的含量明显较高，这表明它们在保护酵母免受高细胞密度发酵的压力中起重要作用。这些发现为根据乙醇发酵过程中接种物密度表征代谢调节和应激反应提供了新的见识。

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