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首页> 外文期刊>Journal of Biotechnology >Identification and expression analysis of miRNAs during batch culture of HEK-293 cells
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Identification and expression analysis of miRNAs during batch culture of HEK-293 cells

机译：HEK-293细胞分批培养过程中miRNA的鉴定与表达分析

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摘要

MicroRNAs (miRNAs) are small non-coding RNAs of about 20-24 nucleotides in length. They regulate gene expression negatively and have been implicated in a wide variety of biological processes. To identify potential miRNAs that may influence the growth and proliferation of mammalian cells cultured in bioreactors, we applied miRNA microarray expression profiling technology to batch cultures of HEK293 cells in protein free media. In our study, we identified miRNAs that were differentially expressed during the exponential and stationary phases, 13 of these showed distinct up regulation trends while 1 exhibited down regulation. These miRNAs have been implicated in cellular differentiation, growth arrest and apoptosis. Specifically, miR-16 and let-7b are potentially useful in the enhancement of bioreactor cell cultures.
机译：微小RNA（miRNA）是长度约为20-24个核苷酸的小型非编码RNA。它们对基因表达负调控，并与多种生物学过程有关。为了鉴定可能影响在生物反应器中培养的哺乳动物细胞的生长和增殖的潜在miRNA，我们将miRNA微阵列表达谱技术应用于无蛋白培养基中的HEK293细胞的分批培养。在我们的研究中，我们确定了在指数期和固定期差异表达的miRNA，其中13种表现出明显的上调趋势，而1种表现出下调。这些miRNA已经涉及细胞分化，生长停滞和细胞凋亡。具体而言，miR-16和let-7b可能在增强生物反应器细胞培养物中有用。

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