Assessing yeast viability from cell size measurements?

Tibayrenc Pierre; Preziosi-Belloy Laurence; Roger Jean-Michel; Ghommidh Charles .

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Assessing yeast viability from cell size measurements?

机译：通过细胞大小评估来评估酵母的生存能力？

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During microbial cell cultures, environmental conditions affect cell physiology and subsequently process efficiency. Physiological changes result in changing cell morphology, such as cell size variations. The aim of this work was to study cell size evolution of a Saccharomyces cerevisiae population exposed to various stresses during alcoholic batch fermentations, and to evaluate the potential use of cell size measurements to infer cell viability. During a reference culture, without perturbation, viability as assessed by propidium iodide staining (PI) remained 100% and mean cell diameter was found to be above 5 mu m. A rapid temperature shift from 33 to 43 degrees C at 50 gl(-1) of ethanol resulted in an immediate arrest of growth and triggered a progressive loss of viability from 100% to 0% and a decrease of mean cell diameter from 5.2 to 3.7 mu m. Cell size distribution curves obtained with a cell counter showed an increasing subpopulation of significantly smaller cells. At single-cell level, combined microscopy size measurements and PI staining showed that this subpopulation was exclusively composed of dead cells. Similar results were obtained after acetic acid or furfural additions. Accordingly, a multivariate data analysis was achieved to estimate the ratio of dead cells from cell size distributions obtained using the cell counter

机译：在微生物细胞培养过程中，环境条件会影响细胞生理，进而影响加工效率。生理变化导致细胞形态变化，例如细胞大小变化。这项工作的目的是研究酒精分批发酵过程中暴露于各种压力下的酿酒酵母种群的细胞大小演变，并评估细胞大小测量在推断细胞活力方面的潜在用途。在参考培养过程中，没有干扰，通过碘化丙啶染色（PI）评估的生存力保持100％，平均细胞直径超过5微米。在50 gl（-1）的乙醇下，温度从33℃迅速转变为43℃，导致生长立即停止，并导致活力从100％逐渐降低到0％，平均细胞直径从5.2降低到3.7亩用细胞计数器获得的细胞大小分布曲线显示明显较小的细胞增加了亚群。在单细胞水平上，结合显微镜尺寸测量和PI染色显示，该亚群完全由死细胞组成。加入乙酸或糠醛后，得到相似的结果。因此，进行了多变量数据分析，以根据使用细胞计数器获得的细胞大小分布估算死细胞的比例。

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《Journal of Biotechnology》 |2010年第2期|共7页
作者
Tibayrenc Pierre; Preziosi-Belloy Laurence; Roger Jean-Michel; Ghommidh Charles .;
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中图分类生物工程学（生物技术）;
关键词
ethanol: 64-17-5; lignocellulosic hydrolysate cell viability; thermal stress; cell diameter; cell size measurement; cell size evolution;

机译：乙醇：64-17-5;木质纤维素水解产物的细胞活力;热应激;细胞直径;细胞大小测量;细胞大小演变;

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1. Assessing yeast viability from cell size measurements? [J] . Tibayrenc Pierre, Preziosi-Belloy Laurence, Roger Jean-Michel, Journal of Biotechnology . 2010,第1a2期

机译：通过细胞大小评估来评估酵母的生存能力？
2. SERS microscopic imaging as novel tool for assessing viability and enumerating yeast cells at various stages of cell cycle in lag, log, exponential and stationary phases of growth in culture [J] . Kiran Manikantan Syamala, Itoh Tamitake, Abe Hiroko, Journal of Experimental Nanoscience . 2014,第10期

机译：SERs微观成像作为评估在细胞周期的各个阶段的可行性和枚举酵母细胞的新型工具，在培养的滞后，对数，指数和固定阶段的各个阶段
3. Comparison of methods used for assessing the viability and vitality of yeast cells [J] . Kwolek-Mirek Magdalena, Zadrag-Tecza Renata FEMS Yeast Research . 2014,第7期

机译：比较评估酵母细胞活力和活力的方法
4. Lensfree on-chip microscopy achieves accurate measurement of yeast cell viability and concentration using machine learning [C] . Alborz Feizi, Yibo Zhang, Alon Greenbaum, Conference on Lasers and Electro-Optics . 2017

机译：Lensfree片上显微镜可以通过机器学习来精确测量酵母细胞的活力和浓度
5. Portable, Cost-effective, and Rapid Yeast Cell Concentration and Viability Measurement using Lensless On-chip Microscopy and Support Vector Machine Classification [D] . Feizi, Alborz. 2016

机译：便携式，经济效益和快速的酵母细胞浓度和使用透镜片上显微镜的可存活率测量，并支持向量机分类
6. Accurate noninvasive measurement of cell size and compartment shape anisotropy in yeast cells using double-PFG MR [O] . Noam Shemesh, Evren Özarslan, Peter J Basser, -1

机译：使用Double-PFG MR准确地在酵母细胞中的细胞尺寸和隔室形状各向异性的非侵入性测量
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机译：恢复辐照酵母细胞的生存能力苏联。

Assessing yeast viability from cell size measurements?

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