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首页> 外文期刊>Journal of Biological Control >A new insect rearing container for in vivo mass multiplication of NPV of Hyblaea puera.
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A new insect rearing container for in vivo mass multiplication of NPV of Hyblaea puera.

机译：一种新的昆虫饲养容器，用于体内大量繁殖Hyblaea puera的NPV。

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摘要

To overcome the problems in rearing the teak defoliator, Hyblaea puera on artificial diet in a two-piece rearing tube for HpNPV mass multiplication, a new three-piece tube was designed and evaluated. The two-piece tube (Tube A) consists of two parts - a cylindrical main body with one end open (55 mm x 23 mm) and a perforated cap. The three-piece tube (Tube B) consists of a main body with both ends open (55 mm x 23 mm), a diet cup (20 mm x 22 mm) and a perforated cap. Comparative evaluation of two types of tubes (main body of Tube A vs Diet cup of Tube B) was made in terms of their virus capture efficiency during inoculation and also easiness in diet dispensing and general handling. The short length of the diet cup of tube B favored highest virus capturing (added 30%) and efficient diet dispensing. Tube B could be an excellent option for rearing H. puera larvae for NPV mass production and laboratory culture maintenance and also for rearing of other lepidopteran larvae of similar behavior and size.
机译：为克服在人工饮食中以两片式饲养管进行HpNPV大量繁殖的情况下以人工饮食饲养柚木落叶树的问题，设计并评估了一种新的三片管。两件式管（管A）由两部分组成-一端开口（55 mm x 23 mm）的圆柱形主体和带孔盖。三件式试管（试管B）由两端开口的主体（55毫米x 23毫米），减肥杯（20毫米x 22毫米）和带孔的盖子组成。对两种类型的试管（试管A的主体与试管B的饮食杯）进行了比较评估，评估了它们在接种过程中的病毒捕获效率，以及饮食分配和一般处理的简便性。 B管的饮食杯的较短长度有利于最高的病毒捕获（增加30％）和有效的饮食分配。 B管可能是饲养H.puera幼虫以进行NPV大规模生产和实验室培养维护的理想选择，也可能是饲养其他行为和大小相似的鳞翅目幼虫的绝佳选择。

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