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首页> 外文期刊>Journal of Biotechnology >A novel RPMXR motif among class II 5-enolpyruvylshikimate-3-phosphate synthases is required for enzymatic activity and glyphosate resistance
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A novel RPMXR motif among class II 5-enolpyruvylshikimate-3-phosphate synthases is required for enzymatic activity and glyphosate resistance

机译：II类5-烯丙基丙酮基shi酸酯-3-磷酸合酶中的新型RPMXR基序对于酶活性和草甘膦抗性是必需的

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摘要

The shikimate pathway enzyme 5-enolpyruvyishikimate-3-phosphate (EPSP) synthase is an attractive target for drugs and herbicides. Here we identified a novel RPMXR motif that is strictly conserved among class II EPSP synthases. Site-directed mutational analysis of this motif showed that substitutions of the four strictly conserved amino acid residues, Arg127, Prol 28, Met129, and Arg131, resulted in complete loss of enzymatic activity, whereas changes in the non-conserved Asn130 residue strongly influenced glyphosate resistance (all numbering according to Pseudomonas stutzeri A1501 EPSP synthase). These experimental results, combined with 3D structure modeling of the location and interaction of the RPMXR motif with phosphoenolpyruvate (PEP) and shikimate-3-phosphate (S3P), demonstrate that the novel motif is required for enzymatic activity and glyphosate resistance of class II EPSP synthases.
机译：sh草酸酯途径酶5-烯醇式丙酮酸草酸酯-3-磷酸酯（EPSP）合酶是药物和除草剂的有吸引力的靶标。在这里，我们确定了一个新的RPMXR基序，该基序在II类EPSP合酶中严格保守。该基序的定点突变分析表明，四个严格保守的氨基酸残基Arg127，Prol 28，Met129和Arg131的取代导致酶活性完全丧失，而非保守Asn130残基的变化强烈影响草甘膦。耐药性（所有编号均根据斯图氏假单胞菌A1501 EPSP合酶编号）。这些实验结果与RPMXR基序与磷酸烯醇丙酮酸（PEP）和and草酸酯-3-磷酸酯（S3P）的位置和相互作用的3D结构建模相结合，证明了II型EPSP的酶活性和草甘膦抗性需要新的基序合酶。

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