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首页> 外文期刊>Journal of Biotechnology >Simultaneous saccharification and fermentation by engineered Saccharomyces cerevisiae without supplementing extracellular beta-glucosidase
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Simultaneous saccharification and fermentation by engineered Saccharomyces cerevisiae without supplementing extracellular beta-glucosidase

机译：工程酿酒酵母同时糖化和发酵，无需补充细胞外β-葡萄糖苷酶

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摘要

Simultaneous saccharification and fermentation (SSF) has been considered a promising and economical process for cellulosic ethanol production. Further cost savings could be gained by reducing enzyme loading and engineering host strain for ethanol production. In this study, we demonstrate efficient ethanol production by SSF without supplementation of beta-glucosidase using an engineered Saccharomyces cerevisiae strain expressing a cellodextrin transporter and an intracellular beta-glucosidase from Neurospora crassa. Ethanol production profiles by the engineered yeast without supplementation of beta-glucosidase and by a parental strain with supplementation of beta-glucosidase were examined under various fermentation conditions. When initial cell mass concentrations were low, the traditional SSF with supplementation of beta-glucosidase showed better ethanol production than SSF with the engineered strain without supplementing beta-glucosidase. However, the engineered strain without supplementation of beta-glucosidase showed almost the same or even better ethanol productivity than the parental strain with supplementation of beta-glucosidase when initial cell mass concentrations were elevated. Our results suggest that efficient ethanol production by SSF could be achieved by engineered yeast capable of fermenting cellobiose without addition of extracellular beta-glucosidase, leading to economic production of cellulosic ethanol
机译：同时糖化和发酵（SSF）被认为是纤维素乙醇生产的一种有前途且经济的方法。通过减少酶的含量和工程生产乙醇的宿主菌株，可以进一步节省成本。在这项研究中，我们证明了由SSF高效生产乙醇，而无需使用表达纤维糊精转运蛋白和胞内β-葡糖苷酶的工程化酿酒酵母菌株从crus Neurospora crassa获得β-葡糖苷酶的补充。在各种发酵条件下，检查了不添加β-葡萄糖苷酶的工程酵母和添加β-葡萄糖苷酶的亲本菌株的乙醇生产状况。当初始细胞质量浓度较低时，传统的补充有β-葡糖苷酶的SSF的乙醇产量要高于不带补充β-葡糖苷酶的工程菌株的SSF。然而，当初始细胞质量浓度升高时，不添加β-葡糖苷酶的工程菌株显示出与添加β-葡糖苷酶的亲本菌株几乎相同或什至更好的乙醇生产率。我们的结果表明，通过能够发酵纤维二糖而无需添加细胞外β-葡糖苷酶的工程酵母可以实现SSF高效生产乙醇，从而经济地生产纤维素乙醇

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