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首页> 外文期刊>Journal of Biotechnology >Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass
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Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass

机译：纤维素酶对木质素吸附的温度敏感性及其对木质纤维素生物质酶解的影响

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摘要

Unproductive enzyme adsorption is an important factor in addition to steric hindrance of lignin that limits the enzymatic hydrolysis of lignocellulosic biomass. While both are important factors, enzymatic hydrolysis of pretreated biomass is most likely conducted in the presence of certain amount of lignin residues that may not necessarily present accessibility hindrance, but can competitively absorb the enzyme. This paper presents a study with purified lignin samples to elucidate the role of unproductive enzyme adsorption. It appeared that lignin adsorbed cellulase quickly at 4 degrees C with adsorption equilibrium reached within 1 h, similar to that observed for crystalline cellulose. Increasing temperature to 50 degrees C (typical hydrolytic reaction condition) facilitated the rate of cellulase adsorption on cellulose with a peak of adsorption reached at 0.25 h; however, adsorption on lignin was surprisingly slower and took over 12 h to reach equilibrium, which was accompanied with a 10-fold increase in adsorption capacity. Despite the high adsorption capacity of lignin (which is comparable to that of cellulose) at 50 degrees C, the presence of added lignin imposed only minimal impact on the enzyme apparent activity, most likely due to the slow adsorption kinetics of lignin
机译：除木质素的空间位阻之外，非生产性酶的吸附是重要的因素，其限制了木质纤维素生物质的酶促水解。尽管两者都是重要因素，但预处理过的生物质的酶促水解最有可能在一定量的木质素残基存在下进行，这些残基不一定会出现可及性障碍，但可以竞争性地吸收酶。本文提出了对纯化的木质素样品的研究，以阐明非生产性酶吸附的作用。似乎木质素在4℃迅速吸附纤维素酶，在1小时内达到吸附平衡，这与结晶纤维素相似。将温度提高到50摄氏度（典型的水解反应条件）有助于纤维素酶在纤维素上的吸附速率达到0.25 h的吸附峰值;然而，木质素的吸附速度出乎意料地慢，需要12小时才能达到平衡，同时吸附能力提高了10倍。尽管木质素在50摄氏度时具有很高的吸附能力（与纤维素相当），但添加木质素的存在对酶的表观活性影响很小，这很可能是由于木质素的缓慢吸附动力学所致。

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