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首页> 外文期刊>Journal of bodywork and movement therapies >Review of Microanalytical in vivo study of biochemical milieu of myofascial trigger points
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Review of Microanalytical in vivo study of biochemical milieu of myofascial trigger points

机译：肌筋膜触发点生化环境的微观分析研究综述

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[Shah, J.P., Phillips, T.M., Danoff, J.V., Gerber, L.H., 2005. An in-vivo microanalytical technique for measuring the local biochemical milieu of human skeletal muscle. Journal of Applied Physiology 99, 1980-1987]: Myofascial pain associated with myofascial trigger points (MTrPs) is a common cause of non-articular musculoskeletal pain. Although the presence of MTrPs can be determined by soft tissue palpation, little is known about the mechanisms and biochemical milieu associated with persistent muscle pain. A microanalytical system was developed to measure the in vivo biochemical milieu of muscle in near real time at the sub-nanogram level of concentration, the system including a microdialysis needle capable of continuously collecting extremely small samples (~0.5 mul) of physiological saline after exposure to the internal tissue milieu across a 105 mum thick semi-permeable membrane. This membrane is positioned 200 mum from the tip of the needle and permits solutes of less than 75kDa to diffuse across it. Three subjects were selected from each of three groups (9 subjects): Normal (no neck pain, no MTrP); Latent (no neck pain, MTrP present); Active (neck pain, MTrP present). The microdialysis needle was inserted in a standardized location in the upper trapezius muscle. Due to the extremely small sample size collected by the microdialysis system, an established micro-analytical laboratory, employing immunoaffinity capillary electrophoresis (ICE) and capillary electrochromatography (CEC), performed analysis of selected analytes. Concentrations of protons (H~+), bradykinin, calcitonin gene-related peptide, substance P, tumor necrosis factor-beta, Interleukin 1-beta, serotonin, and norepinephrine were found to be significantly higher in the Active group than either of the other two groups (P<.01). pH was significantly lower in the Active group than in the other two groups (P<.03). Conclusion: The described microanalytical technique enables continuous sampling of extremely small quantities of substances directly from soft tissue, with minimal system perturbation and without harmful effects on subjects. The measured levels of analytes can be used to distinguish clinically distinct groups.
机译：[Shah，J.P.，Phillips，T.M.，Danoff，J.V.，Gerber，L.H.，2005年。一种体内微分析技术，用于测量人体骨骼肌的局部生化环境。应用生理学杂志99，1980-1987]：与肌筋膜触发点（MTrPs）相关的肌筋膜疼痛是非关节性肌肉骨骼疼痛的常见原因。尽管可以通过软组织触诊确定MTrP的存在，但对与持续性肌肉疼痛相关的机制和生化环境的了解却很少。开发了一种微分析系统，以亚纳米级的浓度几乎实时地测量肌肉的体内生化环境，该系统包括一个微透析针，能够在暴露后连续收集极少量的生理盐水样品（约0.5 mul）穿过105微米厚的半透膜进入内部组织环境。该膜位于距针尖200毫米处，允许小于75kDa的溶质在膜上扩散。从三个组中的每一个中选择三个受试者（9个受试者）：正常（无颈部疼痛，无MTrP）;潜在的（无颈部疼痛，存在MTpP）;活跃（颈部疼痛，存在MTrP）。将微透析针插入斜方肌上部的标准位置。由于微透析系统收集到的样本量极小，因此，一个建立了微分析实验室，采用了免疫亲和毛细管电泳（ICE）和毛细管电色谱（CEC），可以对选定的分析物进行分析。在活动组中，质子（H〜+），缓激肽，降钙素基因相关肽，P物质，肿瘤坏死因子-β，白介素1-β，5-羟色胺和去甲肾上腺素的浓度显着高于其他任何一个两组（P <.01）。活性组的pH显着低于其他两组（P <.03）。结论：所描述的微分析技术能够直接从软组织连续采样极少量的物质，而对系统的干扰最小，并且对受试者没有有害影响。所测量的分析物水平可用于区分临床上不同的组。

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