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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >DNA binding and photocleavage properties of a novel cationic porphyrin-anthraquinone hybrid.
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DNA binding and photocleavage properties of a novel cationic porphyrin-anthraquinone hybrid.

机译：新型阳离子卟啉-蒽醌杂化物的DNA结合和光解特性。

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摘要

A novel cationic porphyrin-anthraquinone (Por-AQ) hybrid has been synthesized and characterized. Using the combination of absorption titration, fluorescence spectra, circular dichroism (CD) as well as viscosity measurements, the binding properties of the hybrid to calf thymus (CT) DNA have been investigated compared with its parent porphyrin. The experimental results show that at low [Por]/[DNA] ratios, the parent porphyrin binds to DNA in an intercalative mode while the hybrid binds in a combined mode of outside binding (for porphyrin moiety) and partial intercalation (for anthraquinone). Ethidium bromide (EB) competition experiment determined the binding affinity constants (K(app)) of the compounds for CT DNA. Theoretical calculational results applying the density functional theory (DFT) can explain the different DNA binding behaviors reasonably. (1)O(2) was suggested to be the reactive species responsible for the DNA photocleavage of porphyrin moieties in both two compounds. The wavelength-dependingcleavage activities of the compounds were also investigated.
机译：合成并表征了新型阳离子卟啉-蒽醌（Por-AQ）杂化物。使用吸收滴定，荧光光谱，圆二色性（CD）以及粘度测量的组合，已经研究了杂种与小牛胸腺（CT）DNA的结合特性（与其母体卟啉相比）。实验结果表明，在低的[Por] / [DNA]比率下，亲本卟啉以嵌入方式与DNA结合，而杂化物以外部结合（对于卟啉部分）和部分嵌入（对蒽醌）的结合方式结合。溴乙锭（EB）竞争实验确定了化合物对CT DNA的结合亲和常数（K（app））。应用密度泛函理论（DFT）的理论计算结果可以合理地解释不同的DNA结合行为。建议（1）O（2）是负责这两个化合物中卟啉部分DNA光解的反应性物种。还研究了化合物的波长依赖性切割活性。

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