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首页> 外文期刊>Journal of Biomechanics >Effect of oscillating fluid flow stimulation on osteocyte mRNA expression
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Effect of oscillating fluid flow stimulation on osteocyte mRNA expression

机译：振荡血流刺激对骨细胞mRNA表达的影响

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摘要

Structural adaptation of the bone tissue is mediated by loading-induced interstitial fluid flow within the bone microstructure. Within this framework, osteocytes fulfill the central mechanotransductive role in the bone remodeling process. While osteocytes have been demonstrated to be exquisitely sensitive to various forms of fluid flow stimulus in vitro, the effect of different oscillating fluid flow (OFF) parameters on osteocyte activity has yet to be systematically characterized. In this study, we investigate the effect of three OFF parameters on osteocyte activity in vitro and hypothesize that COX-2, RANKL, and OPG mRNA expression in osteocytes are sensitive to the OFF parameters: peak shear stress amplitude (0.5. Pa, 1. Pa, 2. Pa, and 5. Pa), oscillating frequency (0.5. Hz, 1. Hz, and 2. Hz), and total flow duration (1. h, 2. h, and 4. h). Our findings demonstrate that COX-2 mRNA levels are elevated in osteocytes subjected to higher peak shear stress amplitudes and longer flow durations, while RANKL/OPG mRNA levels decreased to a minimum threshold in response to higher peak shear stress amplitudes, faster oscillating frequencies, and longer flow durations. These findings suggest that dynamic fluid flow with higher peak shear stress amplitudes, faster oscillating frequencies, and longer loading durations provide the best conditions for promoting bone formation.
机译：骨组织的结构适应由骨微结构内的负载诱导的组织液流动来介导。在此框架内，骨细胞在骨骼重塑过程中起着中心机械转导的作用。虽然已经证明骨细胞对体外的各种形式的流体流动刺激极为敏感，但是尚未有系统地表征不同的振荡流体流动（OFF）参数对骨细胞活性的影响。在这项研究中，我们调查了三个OFF参数对体外骨细胞活性的影响，并假设骨细胞中COX-2，RANKL和OPG mRNA表达对OFF参数敏感：峰值剪切应力振幅（0.5。Pa，1。 Pa，2。Pa和5. Pa），振荡频率（0.5。Hz，1。Hz和2. Hz）和总流量持续时间（1. h，2。h和4. h）。我们的研究结果表明，骨水泥中的COX-2 mRNA水平升高，从而承受较高的峰值剪切应力幅度和较长的流动时间，而RANKL / OPG mRNA水平则降低至最小阈值，以响应较高的峰值剪切应力幅度，更快的振荡频率和持续时间更长。这些发现表明，具有较高的峰值切应力振幅，更快的振荡频率和更长的加载持续时间的动态流体流为促进骨骼形成提供了最佳条件。

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