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首页> 外文期刊>Journal of Biomechanics >Novel mechanical bioreactor for concomitant fluid shear stress and substrate strain
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Novel mechanical bioreactor for concomitant fluid shear stress and substrate strain

机译：新型机械生物反应器，可同时应对流体的切应力和基体应变

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The two main types of mechanical stimuli used in cellular-level bone mechanotransduction studies are substrate strain and flow-induced shear stress. A subset of studies has investigated which of these stimuli induces the primary mechanotransduction effect on bone cells. The shortcomings of these experiments are twofold. First, in some experiments the magnitude of one loading type is able to be quantitatively measured while the other loading mode is only estimated. Second, the two loading modes are compared using different bioreactors, representing different cellular environments and substrates to which the cells are attached. In addition, none of these studies utilized bioreactors which apply controlled magnitudes of substrate strain and flow-induced shear stress differentially and simultaneously. This study presents the design of a multimodal loading device which can apply substrate stretch and fluid flow simultaneously while allowing for real-time cell imaging. The mechanical performance of the bioreactor is validated in this study by correlating the output levels of flow-induced shear stress and substrate strain with the input levels of displacement and displacement rate. The magnitudes of cross-talk loading (i.e. flow-induced strain, and strain-induced fluid flow) are also characterized and shown to be magnitudes lower than physiological levels of loading estimated to occur in bone in vivo.
机译：细胞水平骨机械转导研究中使用的两种主要机械刺激类型是基质应变和流动引起的剪切应力。一部分研究已经研究了这些刺激中的哪些刺激对骨细胞的主要机械转导作用。这些实验的缺点是双重的。首先，在某些实验中，可以定量测量一种加载类型的大小，而仅估算另一种加载模式。其次，使用不同的生物反应器比较两种加载模式，这些反应器代表不同的细胞环境和细胞所附着的基质。此外，这些研究均未使用生物反应器，该反应器可差异且同时施加受控大小的底物应变和流致剪切应力。这项研究提出了一种多模式加载设备的设计，该设备可以同时施加基质拉伸和流体流动，同时允许实时细胞成像。通过将流致剪切应力和基质应变的输出水平与位移和位移速率的输入水平相关联，可以验证生物反应器的机械性能。还表征了串扰负载的大小（即，流动引起的应变和应变引起的流体流动），并显示其大小低于估计在体内骨骼中发生的生理负载水平。

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