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首页> 外文期刊>Journal of Biomechanics >Mechanical stress induces COX-2 mRNA expression in bone cells from elderly women.
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Mechanical stress induces COX-2 mRNA expression in bone cells from elderly women.

机译：机械应力诱导老年女性骨细胞中COX-2 mRNA的表达。

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Mechanical loading-induced fluid flow in the lacuno-canalicular network is a possible signal for bone cell adaptive responses. In an earlier study we found that pulsating fluid flow (PFF, 0.7+/-0.02 Pa, 5 Hz, 0.4 Pa/s) stimulates the production of prostaglandins by neonatal mouse calvarial cells. In addition, mRNA expression of the inducible form of cyclooxygenase (COX-2), but not the constitutive form (COX-1), the major enzymes in prostaglandin production, was increased by PFF. The present study was performed to determine whether human primary bone cells from the iliac crest, respond to mechanical stress in a similar way as neonatal mouse calvarial cells. We subjected bone cells originating from the iliac crest of nine elderly women, between 56 and 80 yr of age, for 1 h to PFF and measured prostaglandin production and COX-1 and COX-2 mRNA expression. One hour PFF treatment stimulated the release of PGE2 by 3.5 fold and PGI2 by 2.2 fold. PFF also increased the expression of COX-2 mRNA by 2.9 fold, but did not change COX-1 mRNA. No correlation was found between donor age and PFF effect, neither on prostaglandin production nor on COX-2 mRNA expression. This study shows that bone cells from the iliac crest of elderly women react to PFF treatment in a similar way as neonatal mouse calvarial cells, namely with increased production of prostaglandins and upregulation of COX-2 mRNA expression. These results suggest that human bone cells from the iliac crest and neonatal mouse calvarial cells share a similar mechanotransduction pathway.
机译：腔-小管网络中机械加载引起的流体流动是骨细胞适应性反应的可能信号。在较早的研究中，我们发现脉动液流（PFF，0.7 +/- 0.02 Pa，5 Hz，0.4 Pa / s）刺激新生小鼠颅盖细胞产生前列腺素。此外，PFF增加了可诱导形式的环氧合酶（COX-2）而不是组成型（COX-1）（前列腺素生产中的主要酶）的mRNA表达。进行本研究以确定来自from的人类原代骨细胞是否以与新生小鼠颅盖细胞类似的方式对机械应力作出反应。我们对来自9名年龄在56至80岁之间的老年女性the骨的骨细胞进行了1小时的PFF检测，并测量了前列腺素的产生以及COX-1和COX-2 mRNA的表达。一小时的PFF处理刺激PGE2释放3.5倍，PGI2释放2.2倍。 PFF还使COX-2 mRNA的表达增加了2.9倍，但没有改变COX-1 mRNA的表达。在供体年龄和PFF作用之间均未发现相关性，既不影响前列腺素的产生也不影响COX-2 mRNA的表达。这项研究表明，老年女性the骨的骨细胞对PFF的反应与新生小鼠颅盖细胞的反应相似，即前列腺素产生增加和COX-2 mRNA表达上调。这些结果表明，来自rest和新生小鼠颅盖细胞的人骨细胞具有相似的机械转导途径。

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